A组链球菌与十六烷液滴的疏水相互作用。
Hydrophobic interactions of group A streptococci with hexadecane droplets.
作者信息
Ofek I, Whitnack E, Beachey E H
出版信息
J Bacteriol. 1983 Apr;154(1):139-45. doi: 10.1128/jb.154.1.139-145.1983.
Abstract
The adherence of Streptococcus pyogenes cells to hexadecane droplets was measured by vortexing water suspensions of streptococci with hexadecane. It was found that adherence of the organisms to hexadecane droplets was abolished by pretreating the organisms with trypsin, pepsin at pH 4.5, or HCl solutions at 95 degrees C. Streptococcal adherence was best expressed in organisms harvested during the stationary phase of growth and was inhibited by fatty acid-free albumin because of the interaction of the protein with the streptococcal surfaces. The data suggest that adherence to hexadecane droplets measures the availability on the surface of S. pyogenes cells of lipophilic residues that are either hydrophobic regions of surface protein structures or, more likely, glycolipids complexed with and oriented by surface proteins.
摘要
通过将酿脓链球菌的水悬浮液与十六烷进行涡旋混合，来测定酿脓链球菌细胞对十六烷液滴的黏附性。结果发现，用胰蛋白酶、pH 4.5的胃蛋白酶或95℃的盐酸溶液预处理这些微生物后，它们对十六烷液滴的黏附性消失。链球菌的黏附性在生长稳定期收获的微生物中表现最佳，且由于无脂肪酸白蛋白与链球菌表面的相互作用，其黏附性受到抑制。数据表明，对十六烷液滴的黏附性测定了酿脓链球菌细胞表面亲脂性残基的可用性，这些亲脂性残基要么是表面蛋白质结构的疏水区域，要么更可能是与表面蛋白质复合并由其定向的糖脂。
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Biochim Biophys Acta. 1963 Nov 29;75:414-24. doi: 10.1016/0006-3002(63)90629-2.
2
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