Poltronieri Palmiro, Primiceri Elisabetta, Radhakrishnan Rajeswaran
CNR-ISPA, Lecce, Italy.
CNR-Nanotec, Lecce, Italy.
Methods Mol Biol. 2019;1918:87-101. doi: 10.1007/978-1-4939-9000-9_7.
In this chapter methods and protocols for surfaces adapted to electrochemical impedance detection, antibody binding, electrolyte couples used, and instrumentation for EIS Biosensing are presented. Various technical bottlenecks have been overcome in recent years. Other limitations still present in this technique are discussed. We present the most recent applications in food pathogen detection based on EIS methods, as well as using other antibody-based platforms.
本章介绍了适用于电化学阻抗检测的表面、抗体结合、所用的电解质对以及电化学阻抗谱生物传感仪器的方法和协议。近年来,各种技术瓶颈已被克服。本文还讨论了该技术中仍然存在的其他局限性。我们介绍了基于电化学阻抗谱方法以及使用其他基于抗体的平台在食品病原体检测中的最新应用。