Rahman Mohammad Azizur, Abdullah Noorlidah, Aminudin Norhaniza
Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1340, Bangladesh.
Saudi J Biol Sci. 2018 Dec;25(8):1515-1523. doi: 10.1016/j.sjbs.2016.01.021. Epub 2016 Feb 8.
Mushrooms have been highly regarded as possessing enormous nutritive and medicinal values. In the present study, we evaluated the anti-oxidative and anti-atherosclerotic potential of shiitake mushroom () using its solvent-solvent partitioned fractions that consisted of methanol:dichloromethane (M:DCM), hexane (HEX), dichloromethane (DCM), ethyl acetate (EA) and aqueous residue (AQ). The hexane fraction (1 mg/mL) mostly scavenged (67.38%, IC 0.55 mg/mL) the 2,2-diphenyl-1-picryl hydrazyl (DPPH) free radical, contained the highest reducing capacity (60.16 mg gallic acid equivalents/g fraction), and most potently inhibited lipid peroxidation (67.07%), low density lipo-protein oxidation and the activity of 3-hydroxy 3-methyl glutaryl co-enzyme A reductase (HMGR). GC-MS analyses of the hexane fraction identified -tocopherol (vitamin E), oleic acid, linoleic acid, ergosterol and butyric acid as the bio-functional components present in Our findings suggest that possesses anti-atherosclerotic bio-functionality that can be applied as functional food-based therapeutics against cardiovascular diseases.
蘑菇一直被高度认为具有巨大的营养和药用价值。在本研究中，我们使用香菇的溶剂 - 溶剂分配级分评估了其抗氧化和抗动脉粥样硬化潜力，这些级分包括甲醇：二氯甲烷（M:DCM）、己烷（HEX）、二氯甲烷（DCM）、乙酸乙酯（EA）和水相残余物（AQ）。己烷级分（1毫克/毫升）对2,2 - 二苯基 - 1 - 苦基肼（DPPH）自由基的清除能力最强（67.38%，IC为0.55毫克/毫升），具有最高的还原能力（60.16毫克没食子酸当量/克级分），并且最有效地抑制脂质过氧化（67.07%）、低密度脂蛋白氧化以及3 - 羟基 - 3 - 甲基戊二酰辅酶A还原酶（HMGR）的活性。对己烷级分的气相色谱 - 质谱分析确定了α - 生育酚（维生素E）、油酸、亚油酸、麦角固醇和丁酸是其中存在的生物功能成分。我们的研究结果表明，香菇具有抗动脉粥样硬化的生物功能，可作为基于功能性食品的心血管疾病治疗剂。
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