Faculty of Biological Sciences, Department of Microbiology and Biotechnology, North Tehran Branch, Islamic Azad University, Tehran, Iran.
Mol Biol Rep. 2022 Jul;49(7):6563-6572. doi: 10.1007/s11033-022-07496-w. Epub 2022 May 10.
Edible-medicinal fungi are mainly used in Asian countries to prevent various diseases. These mushrooms are also used to treat lung diseases and cancer. Ganoderma lucidum and Lentinula edodes are the most important edible-medicinal fungi. The polysaccharides of these fungi are one of the bioactive compounds with anti-cancer properties.
Evaluation of anti-cancer effects of Shiitake and Reishi polysaccharides.
In this study, fungal polysaccharides were extracted using the hot water method and were purified by Diethylaminoethyl Sephadex A-25 (DEAE-Sephadex A-25) chromatography column and their concentration was measured by phenolic sulfuric acid method. The biological effects of the extracted polysaccharides from Ganoderma lucidum and Lentinula edodes on the MCF-7 cell line were investigated using an MTT assay and then its effects on the expression of the P53 cancer regulatory gene and HER-3 gene were investigated.
Based on the results, the concentration of Ganoderma lucidum and Lentinula edodes extracted polysaccharides were 0.024 and 0.103 mg/ml, respectively. Polysaccharides of these two fungi increased the expression of the P53 gene and decreased the expression of the HER-3 gene in a dose and time-dependent manner.
Natural biocompatible polysaccharides with anti-cancer properties that are native, are available, and inexpensive, so they can be used as dietary supplements to prevent and help treat cancer.
食用药用真菌主要在亚洲国家用于预防各种疾病。这些蘑菇也可用于治疗肺部疾病和癌症。灵芝和香菇是最重要的食用药用真菌。这些真菌的多糖是具有抗癌特性的生物活性化合物之一。
评估香菇和灵芝多糖的抗癌作用。
在这项研究中，使用热水法从真菌中提取多糖，并通过二乙基氨基乙基 Sephadex A-25（DEAE-Sephadex A-25）色谱柱对其进行纯化，并通过苯酚-硫酸法测量其浓度。使用 MTT 测定法研究从灵芝和香菇中提取的多糖对 MCF-7 细胞系的生物效应，然后研究其对 P53 癌症调节基因和 HER-3 基因表达的影响。
根据结果，灵芝和香菇提取多糖的浓度分别为 0.024 和 0.103 mg/ml。这两种真菌的多糖以剂量和时间依赖的方式增加了 P53 基因的表达，降低了 HER-3 基因的表达。
天然生物相容性多糖具有抗癌特性，天然存在、可获得且价格低廉，因此可用作膳食补充剂，以预防和帮助治疗癌症。
