光泽精 - 四环素的化学发光及其在原花青素灵敏测定中的应用。
Chemiluminescence of lucigenin-tetracycline and its application for sensitive determination of procyanidin.
作者信息
Quan Shuai, Ji Kaixiang, Liu Fangshuo, Barkae Tesfaye Hailemariam, Halawa Mohamed Ibrahim, Hanif Saima, Lou Baohua, Li Jianping, Xu Guobao
机构信息
College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, PR China.
出版信息
J Food Drug Anal. 2022 Jun 15;30(2):293-302. doi: 10.38212/2224-6614.3411.
Tetracycline was reported to trigger intense lucigenin chemiluminescence (CL). Based on its significant quenching to lucigenin-tetracycline CL, procyanidin was detected. The mechanism of the lucigenin-tetracycline CL and its quenching by procyanidin were proposed. Under the optimum conditions, the calibration curve is linear in the range of 10-1000 nM for procyanidin with a detection limit of 4.4 nM. The RSD for nine replicate determinations of procyanidin was less than 1.7%. Moreover, this method was satisfactorily applied to the determination of procyanidin in human urine samples and health food capsule of grape seed.
据报道，四环素会引发强烈的光泽精化学发光（CL）。基于原花青素对光泽精 - 四环素CL有显著淬灭作用，对其进行了检测。提出了光泽精 - 四环素CL及其被原花青素淬灭的机制。在最佳条件下，原花青素的校准曲线在10 - 1000 nM范围内呈线性，检测限为4.4 nM。原花青素九次重复测定的相对标准偏差小于1.7%。此外，该方法成功应用于人体尿液样本和葡萄籽保健食品胶囊中原花青素的测定。
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