College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province, 712100, China.
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province, 712100, China.
Chemosphere. 2019 Apr;220:137-145. doi: 10.1016/j.chemosphere.2018.12.103. Epub 2018 Dec 17.
The aim of the study was to reduce relative abundance of antibiotic resistant genes (ARGs) in the poultry manure (PM) compost by adding clay and to determine the mechanism of this effect. Five doses of clay additive [at 0% (T1), 2% (T2), 4% (T3), 6% (T4), 8% (T5) and 10% (T6) based on PM dry weight] were compared to explore the mechanism of reduction in ARGs in the PM compost by the addition of clay. The results confirmed that in the initial raw PM from the breeding farm, the ARG concentrations were 1.7-3.01 times higher than that in the domestic PM. High doses of the clay additive play an important role in reduction in the ARGs and are the main factor responsible for significant variations in ARG abundance between the treatment groups. Therefore, we recommend adding high doses of clay (HDC) as an effective means to reduce the maximum percentage of ARGs in PM compost. A heat map correlation study confirmed that HDC addition during the composting process reduced the bioavailable fractions of toxic metals originating from the chicken feed and significantly impacted ARG dispersion.
本研究旨在通过添加粘土来减少家禽粪便（PM）堆肥中抗生素抗性基因（ARGs）的相对丰度，并确定这种效果的机制。比较了五种粘土添加剂剂量[基于 PM 干重的 0%（T1）、2%（T2）、4%（T3）、6%（T4）、8%（T5）和 10%（T6）]，以探索通过添加粘土减少 PM 堆肥中 ARGs 的机制。结果证实，在来自养殖场的原始 PM 中，ARGs 浓度比家庭 PM 高 1.7-3.01 倍。高剂量的粘土添加剂在减少 ARGs 方面发挥了重要作用，是导致处理组之间 ARG 丰度显著变化的主要因素。因此，我们建议添加高剂量的粘土（HDC）作为减少 PM 堆肥中 ARGs 最大百分比的有效手段。热图相关性研究证实，在堆肥过程中添加 HDC 减少了源自鸡饲料的有毒金属的生物可利用部分，并显著影响了 ARG 的分散。
