Department of Chemistry, University of Venda, Private Bag X5050, Thohoyandou 0950, South Africa.
Department of Pharmacy, University of Naples Federico II Via D. Montesano 49, 1-80131 Napoli, Italy.
Molecules. 2018 Dec 21;24(1):39. doi: 10.3390/molecules24010039.
In this study, the chemical profile of a crude methanol extract of Sond was determined by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Column chromatography and preparative thin layer chromatography were used to isolate three indole alkaloids (raucaffricine, -methylsarpagine and spegatrine) and one triterpenoid (lupeol). The antiplasmodial activity was determined using the parasite lactate dehydrogenase (pLDH) assay. The UPLC-MS profile of the crude extract reveals that the major constituents of are raucaffricine (/ 513.2) and spegatrine (/ 352.2). Fraction 3 displayed the highest antiplasmodial activity with an IC of 6.533 μg/mL. However, raucaffricine, isolated from the active fraction did not display any activity. The study identifies the major constituents of and also demonstrates that the major constituents do not contribute to the antiplasmodial activity of .
在这项研究中,采用超高效液相色谱-质谱联用(UPLC-MS)法测定了 Sond 的粗甲醇提取物的化学成分。通过柱色谱和制备薄层层析分离得到三种吲哚生物碱(劳卡非林、β-甲基育亨宾和斯帕金碱)和一种三萜(羽扇豆醇)。采用寄生虫乳酸脱氢酶(pLDH)测定法测定抗疟原虫活性。粗提取物的 UPLC-MS 图谱表明,劳卡非林(/513.2)和斯帕金碱(/352.2)是 Sond 的主要成分。馏分 3 显示出最高的抗疟原虫活性,IC 为 6.533μg/mL。然而,从活性馏分中分离出的劳卡非林没有显示出任何活性。该研究鉴定了 Sond 的主要成分,也表明主要成分不会影响其抗疟原虫活性。