Mojarrab Mahdi, Shiravand Ali, Delazar Abbas, Heshmati Afshar Fariba
Novel Drug Delivery Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah 67346-67149, Iran.
Novel Drug Delivery Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah 67346-67149, Iran ; Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah 67346-67149, Iran.
ScientificWorldJournal. 2014 Jan 14;2014:825370. doi: 10.1155/2014/825370. eCollection 2014.
Ten extracts with different polarity from two Iranian Artemisia species, A. armeniaca Lam. and A. aucheri Boiss, were screened for their antimalarial properties by in vitro β -hematin formation assay. Dichloromethane (DCM) extracts of both plants showed significant antimalarial activities with IC50 values of 1.36±0.01 and 1.83±0.03 mg/mL and IC90 values of 2.12±0.04 and 2.62±0.09 mg/mL for A. armeniaca and A. aucheri, respectively. Bioactivity-guided fractionation of DCM extracts of both plants by vacuum liquid chromatography (VLC) over silica gel with solvent mixtures of increasing polarities afforded seven fractions. Two fractions from DCM extract of A. armeniaca and four fractions from DCM extract of A. aucheri showed potent antimalarial activity with reducing IC50 and IC90 values compared to extracts. The most potent fraction belonged to DCM extract of A. armeniaca with IC50 and IC90 values of 0.47±0.006 and 0.71±0.006 mg/mL, respectively.
通过体外β-血红素形成试验，对来自两种伊朗蒿属植物（亚美尼亚蒿（Artemisia armeniaca Lam.）和奥氏蒿（Artemisia aucheri Boiss））的十种不同极性的提取物进行了抗疟特性筛选。两种植物的二氯甲烷（DCM）提取物均表现出显著的抗疟活性，亚美尼亚蒿和奥氏蒿的IC50值分别为1.36±0.01和1.83±0.03mg/mL，IC90值分别为2.12±0.04和2.62±0.09mg/mL。通过硅胶柱上的真空液相色谱（VLC），使用极性递增的溶剂混合物对两种植物的DCM提取物进行生物活性导向分级分离，得到了七个级分。与提取物相比，亚美尼亚蒿DCM提取物的两个级分和奥氏蒿DCM提取物的四个级分显示出较强的抗疟活性，其IC50和IC90值降低。最有效的级分属于亚美尼亚蒿的DCM提取物，IC50和IC90值分别为0.47±0.006和0.71±0.006mg/mL。
