promotes proliferation, migration, and invasion of MG63 cells by upregulation of via negatively regulating .

PURPOSE

This article aimed to investigate the mechanism by which and affected the development of osteosarcoma.

METHODS

Tumor tissues and adjacent tissues of 23 osteosarcoma patients were collected. Normal osteoblasts hFOB1.19 and osteosarcoma cells MG63 were cultured. MG63 cells were transfected and grouped: si-negative control (NC) group, si-MALAT1 group, miR-129 NC group, miR-129 mimics group, p-Empty vector group, p-MALAT1 group, p-MALAT1+ miR-129 mimics group, and p-MALAT1+ si-TGIF2 group. Luciferase reporter assay, Cell Counting Kit-8 assay, Transwell assay, quantitative reverse transcription PCR, Western blot, and Pearson correlation analysis were performed.

RESULTS

expression in tumor tissues and MG63 cells was increased (<0.01). High expression predicted poor prognosis of osteosarcoma patients. MG63 cells of si-MALAT1 group exhibited much lower cell viability, migration, and invasive cell numbers when compared with si-NC group (<0.01). For MG63 cells of miR-129 mimics group, they had markedly lower cell viability, migration, and invasive cell numbers than miR-129 NC group (<0.01). was targetedly and negatively regulated by . , which was targetedly and negatively regulated by , was overexpressed in tumor tissues and MG63 cells (<0.01). overexpresison and downregulation significantly reversed the enhanced cell viability, migration, and invasion induced by (<0.01).

CONCLUSION

promotes expression through negative regulation of , which further promotes the proliferation, migration, and invasion of MG63 cells.