Institute of Extreme Environment Nutrition and Protection, Harbin Institute of Technology, Harbin, China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, Harbin Institute of Technology, Harbin, China.
Institute of Extreme Environment Nutrition and Protection, Harbin Institute of Technology, Harbin, China.
Int J Biol Macromol. 2019 Jul 15;133:911-919. doi: 10.1016/j.ijbiomac.2019.04.101. Epub 2019 Apr 15.
A water-soluble polysaccharide was isolated from marine green algae Ulva pertusa and then chelated with iron to prepare the polysaccharide‑iron (III) complex. The immunomodulatory activities of sulfated polysaccharide and polysaccharide‑iron (III) complex were investigated through a mice immune-deficiency model. Cyclophosphamide (Cy) was utilized to establish mice immunodeficiency model. Both polysaccharide and polysaccharide‑iron (III) complex were proved to promote the proliferation of lymphocyte and enhance the activities of mice macrophages. In mice serum, the levels of cytokines including TNF-α, IFN-γ and IL-10 restored and the contents of hemolysin were also found elevated after treatment with polysaccharide and its iron complex. Besides, it has been shown that both polysaccharide and polysaccharide‑iron (III) complex increased the contents of Hb, RBC and HCT in mice blood, and the effect of iron complex was better. All these results suggested that Ulva pertusa polysaccharide could be developed as a healthy function food. It was also noteworthy that the polysaccharide‑iron (III) complex showed no negative effect upon the immunomodulatory activity of polysaccharide. Instead, the polysaccharide‑iron (III) complex showed excellent hematopoietic capacity perhaps due to the supplement of iron.
从海洋绿藻石莼中分离得到一种水溶性多糖，然后用铁螯合，制备多糖-铁（III）复合物。通过建立免疫缺陷小鼠模型，研究了硫酸化多糖和多糖-铁（III）复合物的免疫调节活性。环磷酰胺（Cy）用于建立免疫缺陷小鼠模型。结果表明，多糖和多糖-铁（III）复合物均能促进淋巴细胞增殖，增强小鼠巨噬细胞的活性。在小鼠血清中，多糖及其铁复合物处理后，TNF-α、IFN-γ 和 IL-10 等细胞因子的水平得到恢复，溶血素的含量也升高。此外，多糖和多糖-铁（III）复合物均能提高小鼠血液中 Hb、RBC 和 HCT 的含量，且铁复合物的效果更好。所有这些结果表明，石莼多糖可作为一种具有健康功能的食品。值得注意的是，多糖-铁（III）复合物对多糖的免疫调节活性没有负面影响，反而由于铁的补充，表现出优异的造血能力。
