Overby Robyn J, Feldman Dale S
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
J Funct Biomater. 2018 Dec 24;10(1):1. doi: 10.3390/jfb10010001.
Chronic dermal lesions, such as pressure ulcers, are difficult to heal. Degradable tissue scaffold systems can be employed to serve as a provisional matrix for cellular ingrowth and facilitate regenerative healing during degradation. Degradable regenerative tissue scaffold matrices can be created by crosslinking albumin with functionalized poly(ethylene glycol) (PEG) polymers. The purpose of this study was to evaluate the stability of PEG-albumin scaffold systems formed using PEG polymers with three different functionalized end chemistries by quantifying in vitro system swellability to determine the most promising PEG crosslinking polymer for wound healing applications. Of the three polymers evaluated, PEG-succinimidyl glutarate (SG) exhibited consistent gelation and handling characteristics when used as the crosslinking agent with albumin. PEG-SG polymers were identified as an appropriate synthetic crosslinking moiety in a PEG-albumin scaffold system, and further in vitro and in vivo evaluation of this scaffold system is merited.
慢性皮肤损伤，如压疮，难以愈合。可降解组织支架系统可作为细胞向内生长的临时基质，并在降解过程中促进再生愈合。可通过将白蛋白与功能化聚乙二醇（PEG）聚合物交联来制备可降解再生组织支架基质。本研究的目的是通过量化体外系统膨胀性，评估使用具有三种不同功能化端基化学的PEG聚合物形成的PEG-白蛋白支架系统的稳定性，以确定最有前景的用于伤口愈合应用的PEG交联聚合物。在评估的三种聚合物中，聚乙二醇琥珀酰亚胺戊二酸酯（PEG-SG）用作白蛋白交联剂时表现出一致的凝胶化和操作特性。PEG-SG聚合物被确定为PEG-白蛋白支架系统中合适的合成交联部分，值得对该支架系统进行进一步的体外和体内评估。
