Giliomee Johnel, du Toit Lisa C, Kumar Pradeep, Klumperman Bert, Choonara Yahya E
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, 7 York Road, Parktown 2193, South Africa.
Department of Chemistry and Polymer Science, Faculty of Science, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa.
Polymers (Basel). 2021 May 31;13(11):1828. doi: 10.3390/polym13111828.
In this study, the effect of crosslinking and concentration on the properties of a new library of low-concentration poly(Lys--Ala)-based hydrogels for potential application in wound healing was investigated in order to correlate the hydrogel composition with the desired physicochemical and biofunctional properties to expand the assortment of poly-l-lysine (PLL)-based hydrogels suitable for wound healing. Controlled ring-opening polymerization (ROP) and precise hydrogel compositions were used to customize the physicochemical and biofunctional properties of a library of new hydrogels comprising poly(l-lysine--l-alanine) and four-arm poly(ethylene glycol) (P(KA)/4-PEG). The chemical composition and degree of crosslinking via free amine quantification were analyzed for the P(KA)/4-PEG hydrogels. In addition, the rheological properties, pore morphology, swelling behavior and degradation time were characterized. Subsequently, in vitro cell studies for evaluation of the cytotoxicity and cell adhesion were performed. The 4 wt% 1:1 functional molar ratio hydrogel with P(KA) concentrations as low as 0.65 wt% demonstrated low cytotoxicity and desirable cell adhesion towards fibroblasts and thus displayed a desirable combination of properties for wound healing application.
在本研究中，研究了交联和浓度对一种新型低浓度聚（赖氨酸-丙氨酸）基水凝胶性能的影响，该水凝胶有望应用于伤口愈合，目的是将水凝胶组成与所需的物理化学和生物功能特性相关联，以扩大适用于伤口愈合的聚-L-赖氨酸（PLL）基水凝胶的种类。采用可控开环聚合（ROP）和精确的水凝胶组成来定制由聚（L-赖氨酸-L-丙氨酸）和四臂聚乙二醇（P(KA)/4-PEG）组成的新型水凝胶库的物理化学和生物功能特性。通过游离胺定量分析了P(KA)/4-PEG水凝胶的化学组成和交联度。此外，还对其流变性能、孔形态、溶胀行为和降解时间进行了表征。随后，进行了体外细胞研究以评估细胞毒性和细胞粘附。具有低至0.65 wt% P(KA)浓度的4 wt% 1:1功能摩尔比水凝胶对成纤维细胞表现出低细胞毒性和理想的细胞粘附性，因此显示出适用于伤口愈合应用的理想性能组合。
