Federal Research and Clinical Centre of Physical-Chemical Medicine, Malaya Pirogovskaya 1a, Moscow, 119435, Russia.
Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region, Russian Federation, 141700.
BMC Genomics. 2018 Dec 27;19(1):968. doi: 10.1186/s12864-018-5306-5.
Crohn's disease is associated with gut dysbiosis. Independent studies have shown an increase in the abundance of certain bacterial species, particularly Escherichia coli with the adherent-invasive pathotype, in the gut. The role of these species in this disease needs to be elucidated.
We performed a metagenomic study investigating the gut microbiota of patients with Crohn's disease. A metagenomic reconstruction of the consensus genome content of the species was used to assess the genetic variability.
The abnormal shifts in the microbial community structures in Crohn's disease were heterogeneous among the patients. The metagenomic data suggested the existence of multiple E. coli strains within individual patients. We discovered that the genetic diversity of the species was high and that only a few samples manifested similarity to the adherent-invasive varieties. The other species demonstrated genetic diversity comparable to that observed in the healthy subjects. Our results were supported by a comparison of the sequenced genomes of isolates from the same microbiota samples and a meta-analysis of published gut metagenomes.
The genomic diversity of Crohn's disease-associated E. coli within and among the patients paves the way towards an understanding of the microbial mechanisms underlying the onset and progression of the Crohn's disease and the development of new strategies for the prevention and treatment of this disease.
克罗恩病与肠道菌群失调有关。独立研究表明，肠道中某些细菌物种的丰度增加，特别是具有黏附侵袭表型的大肠杆菌。这些物种在这种疾病中的作用需要阐明。
我们进行了一项宏基因组研究，调查了克罗恩病患者的肠道微生物群。使用物种共识基因组内容的宏基因组重建来评估遗传变异性。
克罗恩病患者的微生物群落结构异常变化具有异质性。宏基因组数据表明，个体患者体内存在多种大肠杆菌菌株。我们发现该物种的遗传多样性很高，只有少数样本与黏附侵袭变种相似。其他物种表现出与健康受试者相当的遗传多样性。我们的结果得到了来自相同微生物群落样本的分离株测序基因组的比较和已发表的肠道宏基因组的荟萃分析的支持。
克罗恩病相关大肠杆菌在患者内和患者间的基因组多样性为理解克罗恩病发病和进展的微生物机制以及开发预防和治疗这种疾病的新策略铺平了道路。
