Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
Section of Molecular Biology, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
Syst Appl Microbiol. 2019 May;42(3):284-290. doi: 10.1016/j.syapm.2018.12.004. Epub 2018 Dec 14.
A group of thirteen bacterial strains was isolated from rock samples collected in a deglaciated northern part of James Ross Island, Antarctica. The cells were rod-shaped, Gram-stain-negative, non-motile, catalase positive, and produced moderately slimy, ultraviolet light (UVC)-irradiation-resistant and red-pink pigmented colonies on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, extensive biotyping, fatty acid profile, chemotaxonomy analyses, and whole genome sequencing were applied in order to clarify the taxonomic position of these isolates. Phylogenetic analysis based on the 16S rRNA gene indicated that all isolates constituted a coherent group belonging to the genus Hymenobacter. The closest relatives to the representative isolate P5136 were Hymenobacter psychrophilus BZ33r and Hymenobacter rubripertinctus CCM 8852, exhibiting 97.53% and 97.47% 16S rRNA pairwise similarity, respectively. Average nucleotide identity calculated from the whole-genome sequencing data supported the finding that P5136 represents a distinct Hymenobacter species. The major components in fatty acid profiles were Summed Feature 3 (Cω7c/Cω6c), Cω5c, C iso and C anteiso. The cellular quinone content contained unanimously menaquinone MK-6 and MK-7 (ratio 1:5.1). The predominant polar lipid was phosphatidylethanolamine, and moderate to minor amounts of two unknown polar lipids, two unknown aminolipids, one unknown glycolipid and two unknown glycophospholipids were present. The G+C content of genomic DNAs is 60.31mol%. Based on all the obtained results, we propose a novel species for which the name Hymenobacter amundsenii sp. nov. is suggested, with the type strain P5136 (=CCM 8682=LMG 29687).
从南极洲詹姆斯·罗斯岛的冰川消退地区采集的岩石样本中分离出了 13 株细菌。这些细胞呈杆状，革兰氏阴性，不运动，过氧化氢酶阳性，在 R2A 琼脂上产生中度粘稠、耐紫外线（UVC）照射和粉红色色素的菌落。应用基于 16S rRNA 基因测序、广泛的生物分型、脂肪酸图谱、化学分类学分析和全基因组测序的多相分类方法来阐明这些分离物的分类地位。基于 16S rRNA 基因的系统发育分析表明，所有分离物构成一个属于海姆杆菌属的一致群体。代表菌株 P5136 与 Hymenobacter psychrophilus BZ33r 和 Hymenobacter rubripertinctus CCM 8852 的亲缘关系最近，其 16S rRNA 序列相似度分别为 97.53%和 97.47%。全基因组测序数据计算的平均核苷酸同一性支持了 P5136 代表一个独特的海姆杆菌种的发现。脂肪酸图谱中的主要成分是 Summed Feature 3（Cω7c/Cω6c）、Cω5c、C iso 和 C anteiso。细胞醌类含有一致的menaquinone MK-6 和 MK-7（比例 1:5.1）。主要的极性脂是磷脂酰乙醇胺，还有中等至少量的两种未知极性脂、两种未知胺脂、一种未知糖脂和两种未知糖磷脂。基因组 DNA 的 G+C 含量为 60.31mol%。基于所有获得的结果，我们提出了一个新的物种，建议使用 P5136（=CCM 8682=LMG 29687）作为模式菌株，并将其命名为 Hymenobacter amundsenii sp. nov.。
