Sedláček Ivo, Králová Stanislava, Kýrová Kamila, Mašlaňová Ivana, Busse Hans-Jürgen, Staňková Eva, Vrbovská Veronika, Němec Miroslav, Barták Miloš, Holochová Pavla, Švec Pavel, Pantůček Roman
Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
Section of Genetics and Molecular Biology, Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic.
Int J Syst Evol Microbiol. 2017 Jun;67(6):1975-1983. doi: 10.1099/ijsem.0.001898. Epub 2017 Jun 20.
Four rod-shaped and Gram-stain-negative bacterial strains, CCM 8647, CCM 8649T, CCM 8643T and CCM 8648T, were isolated from rock samples collected on James Ross Island, Antarctica. Extensive biotyping, fatty acid profiling, chemotaxonomy, 16S rRNA gene sequencing and whole-genome sequencing was applied to isolates to clarify their taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that all four isolates belonged to the genus Hymenobacter. Strains CCM 8649T and CCM 8647 were most closely related to Hymenobacter arizonensis OR362-8T (94.4 % 16S rRNA gene sequence similarity), strain CCM 8643T to Hymenobacter terrae DG7AT (96.3 %) and strain CCM 8648T to Hymenobacter glaciei VUG-A130T (96.3 %). The predominant fatty acids of CCM 8649T and CCM 8647 were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), C16 : 1ω5c and iso-C15 : 0, whereas those of CCM 8643T and CCM 8648T were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and C16 : 1ω5c. The quinone systems contained exclusively menaquinone MK-7. The major polyamine was sym-homospermidine. All four strains contained the major polar lipid phosphatidylethanolamine. The G+C content of genomic DNA ranged from 60-63 mol%. Whole-genome sequencing data supported the finding that isolates represented distinct species of the genus Hymenobacter. On the basis of the results obtained, three novel species are proposed for which the names Hymenobacter coccineus sp. nov., Hymenobacter lapidarius sp. nov. and Hymenobacter glacialis sp. nov. are suggested, with the type strains CCM 8649T (=LMG 29441T=P5239T), CCM 8643T (=LMG 29435T=P3150T) and CCM 8648T (=LMG 29440T=P5086T), respectively.
从南极洲詹姆斯罗斯岛采集的岩石样本中分离出四株杆状革兰氏阴性细菌菌株，即CCM 8647、CCM 8649T、CCM 8643T和CCM 8648T。对这些分离株进行了广泛的生物分型、脂肪酸谱分析、化学分类学、16S rRNA基因测序和全基因组测序，以明确它们的分类地位。基于16S rRNA基因测序的系统发育分析表明，所有四株分离株均属于嗜皮菌属。菌株CCM 8649T和CCM 8647与亚利桑那嗜皮菌OR362-8T关系最为密切（16S rRNA基因序列相似性为94.4%），菌株CCM 8643T与土壤嗜皮菌DG7AT关系密切（96.3%），菌株CCM 8648T与冰川嗜皮菌VUG-A130T关系密切（96.3%）。CCM 8649T和CCM 8647的主要脂肪酸为总和特征3（C16 : 1ω7c/C16 : 1ω6c）、C16 : 1ω5c和异-C15 : 0，而CCM 8643T和CCM 8648T的主要脂肪酸为总和特征3（C16 : 1ω7c/C16 : 1ω6c）和C16 : 1ω5c。醌系统仅含甲基萘醌MK-7。主要的多胺是对称高亚精胺。所有四株菌株均含有主要的极性脂质磷脂酰乙醇胺。基因组DNA的G+C含量范围为60-63mol%。全基因组测序数据支持这些分离株代表嗜皮菌属不同物种的发现。根据所得结果，提出了三个新物种，分别命名为红色嗜皮菌（Hymenobacter coccineus sp. nov.）、石生嗜皮菌（Hymenobacter lapidarius sp. nov.）和冰川嗜皮菌（Hymenobacter glacialis sp. nov.），其模式菌株分别为CCM 8649T（=LMG 29441T=P5239T）、CCM 8643T（=LMG 29435T=P3150T）和CCM 8648T（=LMG 29440T=P5086T）。
