Abdrabou Mohamed I, Elleithy Ebtihal M M, Yasin Noha A E, Shaheen Youssef M, Galal Mona
Department of Cytology and Histology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Department of Biochemistry, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Acta Histochem. 2019 Feb;121(2):198-206. doi: 10.1016/j.acthis.2018.12.006. Epub 2018 Dec 24.
The present study was conducted to evaluate the ameliorative effects of Allium sativum (garlic) as well as Spirulina maxima on lead acetate toxicity in rat testes. Forty adult, male, albino rats were divided into four groups (10 rats/each): group I served as the control; group II contained rats that received lead acetate (100 mg/kg); group III contained rats that received both lead acetate (100 mg/kg) and garlic (600 mg/kg); and group IV contained rats that received both lead acetate (100 mg/kg) and spirulina (500 mg/kg). All treatments were performed daily for one month. Serum testosterone levels, oxidative stress parameters, expression of the caspase-3 gene and histological, histo-morphometric and ultrastructure alterations in the testes were investigated. The results revealed that the Pb-treated group exhibited a significant increase in MDA concentration concomitantly with a decrease in serum testosterone levels, antioxidative marker levels and caspase-3 gene expression. Several histological and histo-morphometric alterations were observed in this group. Co-administration with spirulina or garlic caused a significant increase in testosterone levels, testicular SOD and CAT activities, and caspase-3 gene expression and a decrease in MDA levels, with improvement in histological and histo-morphometric alterations. These results suggested that spirulina was more effective at providing protection against Pb-induced reproductive damage in rats than garlic, indicating the beneficial role of spirulina in improvement of spermatogenesis and steroidogenesis after lead exposure.
本研究旨在评估大蒜和极大螺旋藻对醋酸铅诱导的大鼠睾丸毒性的改善作用。将40只成年雄性白化大鼠分为四组（每组10只）：第一组作为对照组；第二组大鼠接受醋酸铅（100 mg/kg）；第三组大鼠接受醋酸铅（100 mg/kg）和大蒜（600 mg/kg）；第四组大鼠接受醋酸铅（100 mg/kg）和螺旋藻（500 mg/kg）。所有处理每天进行，持续一个月。检测血清睾酮水平、氧化应激参数、caspase-3基因表达以及睾丸的组织学、组织形态计量学和超微结构变化。结果显示，醋酸铅处理组的丙二醛（MDA）浓度显著升高，同时血清睾酮水平、抗氧化标志物水平和caspase-3基因表达降低。该组出现了一些组织学和组织形态计量学改变。与螺旋藻或大蒜共同给药可使睾酮水平、睾丸超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性以及caspase-3基因表达显著升高，MDA水平降低，组织学和组织形态计量学改变得到改善。这些结果表明，在保护大鼠免受醋酸铅诱导的生殖损伤方面，螺旋藻比大蒜更有效，这表明螺旋藻在铅暴露后改善精子发生和类固醇生成方面具有有益作用。
