Jinan Perfect Biological Technology Co. Ltd., Jinan, China.
Department of Orthopaedics, The First Affiliated Hospital of Shandong First Medical University, Jinan, China.
Cell Transplant. 2024 Jan-Dec;33:9636897231219395. doi: 10.1177/09636897231219395.
Lead acetate can cause testicular damage in males. In this study, we assessed the repairing effects of human umbilical cord mesenchymal stem cells (MSCs) on testicular injury caused by lead acetate in mice. MSCs were injected into mice with testicular injury by intraperitoneal injection, and the organ coefficient of reproductive organs, sperm motility, hormone level and antioxidant index of mice were tested. Compared with the normal group, the coefficient of reproductive organs and sperm motility were reduced in the model group, and histopathology showed obvious testicular injury, proving successful modeling. Compared with the model group, the reproductive organ coefficient and sperm motility were improved in the experimental group, and histopathology showed that the testicular injury could be significantly improved. Sex hormone secretion tends to be normal, and the antioxidant index increased. Sequencing results showed that there were 485 upregulated genes and 172 downregulated genes between the model group and the control group, and 210 upregulated genes and 482 downregulated genes between the experimental group and the model group. Differentially expressed genes are mainly concentrated in AMP-activated protein kinase (AMPK) signaling pathway, apoptosis signaling pathway, and arginine biosynthesis signaling pathway. Overall, MSCs can significantly improve the degree of damages to mice testis caused by lead acetate and have a certain repairing effect.
醋酸铅可导致雄性动物睾丸损伤。在这项研究中，我们评估了人脐带间充质干细胞（MSCs）对醋酸铅致小鼠睾丸损伤的修复作用。通过腹腔注射将 MSCs 注入睾丸损伤的小鼠体内，检测小鼠生殖器官器官系数、精子活力、激素水平和抗氧化指标。与正常组相比，模型组生殖器官系数和精子活力降低，组织病理学显示明显的睾丸损伤，证明建模成功。与模型组相比，实验组生殖器官系数和精子活力得到改善，组织病理学显示睾丸损伤可得到明显改善。性激素分泌趋于正常，抗氧化指标增加。测序结果显示，模型组与对照组之间有 485 个上调基因和 172 个下调基因，实验组与模型组之间有 210 个上调基因和 482 个下调基因。差异表达基因主要集中在 AMP 激活的蛋白激酶（AMPK）信号通路、细胞凋亡信号通路和精氨酸生物合成信号通路。总之，MSCs 可显著改善醋酸铅致小鼠睾丸损伤的程度，具有一定的修复作用。
