Department of Chemistry, University of Turin, Via Giuria, 5, I-10125 Turin, Italy.
Biosensors (Basel). 2018 Dec 26;9(1):2. doi: 10.3390/bios9010002.
Simultaneous measurement of different substances from a single sample is an emerging issue for achieving efficient and high-throughput detection in several fields of application. Although immunoanalytical techniques have well-established and prevailing advantages over alternative screening analytical platforms, one of the incoming challenges for immunoassay is exact multiplexing. Lateral flow immunoassay (LFIA) is a leading immunoanalytical technique for onsite analysis, thanks to its simplicity, rapidity, and cost-effectiveness. Moreover, LFIA architecture is adaptable to multiplexing, and is therefore a possible answer to the pressing demand of multiplexing point-of-need analysis. This review presents an overview of diverse approaches for multiplex LFIA, with a special focus on strategies based on new types of magnetic, fluorescent, and colored labels.
同时测量来自单一样本的不同物质是在多个应用领域实现高效和高通量检测的一个新兴问题。尽管免疫分析技术在替代筛选分析平台方面具有成熟和普遍的优势，但免疫分析面临的一个新挑战是精确的多重分析。侧向流动免疫分析（LFIA）是一种用于现场分析的领先免疫分析技术，这要归功于其简单、快速和经济高效的特点。此外，LFIA 结构可适应多重分析，因此是满足多点即时分析对多重分析的迫切需求的一种可能的解决方案。本文综述了多种用于多重 LFIA 的方法，特别关注基于新型磁性、荧光和有色标记的策略。
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