Sun Yaning, Yang Jifei, Yang Suzhen, Sang Qingbo, Teng Man, Li Qingmei, Deng Ruiguang, Feng Lili, Hu Xiaofei, Zhang Gaiping
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences Zhengzhou 450002 China
Zhengzhou Ruilong Food Science and Technology Limited Company Zhengzhou 450064 China.
RSC Adv. 2018 Mar 5;8(17):9580-9586. doi: 10.1039/c8ra01116h. eCollection 2018 Feb 28.
A colloidal gold-based immunochromatographic strip with a competitive format has been developed for the rapid, simultaneous, semi-quantitative and quantitative detection of several aminoglycoside residues in milk, including gentamicin sulfate (GM), neomycin sulfate (NEO) and kanamycin sulfate (KN). Three monoclonal antibodies against the three corresponding aminoglycosides were conjugated to colloidal gold particles and applied to the conjugate pads of the strip. The competitors [GM-bovine serum albumin (GM-BSA), NEO-BSA and KN-BSA conjugates] of GM, NEO and KN were immobilized onto a nitrocellulose (NC) membrane at three detection zones, T1, T2, and T3, respectively. The minimal cut-off values of the strip were 10 ng mL for GM, and 100 ng mL for NEO and KN, which are lower than the maximum residue levels (MRLs) established for aminoglycosides. The IC values of the strip were 0.737 ng mL, 8.971 ng mL and 11.110 ng mL for GM, NEO and KN respectively. In conclusion, the immunochromatographic lateral flow strip could be used for rapid, simultaneous, semi-quantitative and quantitative detection of GM, NEO and KN residues in milk.
一种基于胶体金的竞争型免疫层析试纸条已被开发出来，用于快速、同时、半定量和定量检测牛奶中的几种氨基糖苷类残留，包括硫酸庆大霉素（GM）、硫酸新霉素（NEO）和硫酸卡那霉素（KN）。针对三种相应氨基糖苷类的三种单克隆抗体与胶体金颗粒偶联，并应用于试纸条的偶联垫。GM、NEO和KN的竞争物[GM-牛血清白蛋白（GM-BSA）、NEO-BSA和KN-BSA偶联物]分别固定在硝酸纤维素（NC）膜的三个检测区T1、T2和T3上。试纸条的最低检测限为GM 10 ng/mL，NEO和KN为100 ng/mL，低于为氨基糖苷类规定的最大残留限量（MRLs）。试纸条对GM、NEO和KN的IC值分别为0.737 ng/mL、8.971 ng/mL和11.110 ng/mL。总之，免疫层析侧向流动试纸条可用于快速、同时、半定量和定量检测牛奶中的GM、NEO和KN残留。
