Wang Shiqiong, Li Haihua, Du Chenhong, Liu Qian, Yang Dongji, Chen Longbin, Zhu Qi, Wang Zhixiang
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, China.
PeerJ. 2018 Dec 18;6:e6060. doi: 10.7717/peerj.6060. eCollection 2018.
supplementation is beneficial to the barrier function of the intestinal physical barrier in piglets. However, the mechanisms underlying this beneficial function remain largely unknown. Here, we investigated the effects of dietary supplementation with on the performance, intestinal physical barrier functioning, and NOD-like receptors (NLRs) expression in weaned piglets. Sixteen weaned piglets were randomly allocated to two groups. The control group received a corn-soybean basal diet, while the treatment group received the same diet adding 0.1% , for 14 days. As a result, dietary supplementation was found to increase the average daily gain (ADG) ( < 0.05), reduced serum diamine oxidase (DAO) activity ( < 0.05), increased the mRNA expression and protein abundance of occludin in the jejunum and ileum ( < 0.01), reduced the mRNA levels of NOD1 ( < 0.01), receptor interacting serine/threonine kinase 2 (RIPK2) ( < 0.05), nuclear factor κB (NF-κB) ( < 0.01), NLR family pyrin domain containing 3 (NLRP3) ( < 0.01), caspase-1 ( < 0.01), interleukin 1β (IL-1β) ( < 0.05) and IL-18 ( < 0.01) in the jejunum tissues of the weaned pigs. The expression of NLRP3 ( < 0.05), caspase-1 ( < 0.01), IL-1β ( < 0.05) and IL-18 ( < 0.05) was also reduced in the ileum tissues of the weaned pigs. These results showed that supplementation improves the growth performance, enhances the intestinal physical barrier function, and inhibits the expression of NOD1 and NLRP3 signaling-pathway-related genes in jejunum and ileum tissues. They also suggest that enhances the intestinal physical barrier functioning by inhibiting IL-1β and IL-18 pro-inflammatory cytokines via the NOD1/NLRP3 signaling pathway in weaned piglets.
补充剂对仔猪肠道物理屏障的屏障功能有益。然而，这种有益功能背后的机制在很大程度上仍不清楚。在此，我们研究了日粮添加[具体物质未给出]对断奶仔猪生长性能、肠道物理屏障功能以及NOD样受体（NLRs）表达的影响。16头断奶仔猪被随机分为两组。对照组饲喂玉米 - 大豆基础日粮，而处理组在相同日粮中添加0.1%的[具体物质未给出]，持续14天。结果发现，日粮添加[具体物质未给出]可提高平均日增重（ADG）（P < 0.05），降低血清二胺氧化酶（DAO）活性（P < 0.05），增加空肠和回肠中闭合蛋白的mRNA表达和蛋白丰度（P < 0.01），降低断奶仔猪空肠组织中NOD1（P < 0.01）、受体相互作用丝氨酸/苏氨酸激酶2（RIPK2）（P < 0.05）、核因子κB（NF - κB）（P < 0.01）、NLR家族含pyrin结构域蛋白3（NLRP3）（P < 0.01）、半胱天冬酶 - 1（caspase - 1）（P < 0.01）、白细胞介素1β（IL - 1β）（P < 0.05）和白细胞介素18（IL - 18）（P < 0.01）的mRNA水平。断奶仔猪回肠组织中NLRP3（P < 0.05）、caspase - 1（P < 0.01）、IL - 1β（P < 0.05）和IL - 18（P < 0.05）的表达也降低。这些结果表明，添加[具体物质未给出]可改善生长性能，增强肠道物理屏障功能，并抑制空肠和回肠组织中NOD1和NLRP3信号通路相关基因的表达。它们还表明，[具体物质未给出]通过断奶仔猪中NOD1/NLRP3信号通路抑制IL - 1β和IL - 18促炎细胞因子来增强肠道物理屏障功能。
