绞股蓝多糖降血糖活性评价。
Anti-diabetic activity evaluation of a polysaccharide extracted from Gynostemma pentaphyllum.
机构信息
College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
出版信息
Int J Biol Macromol. 2019 Apr 1;126:209-214. doi: 10.1016/j.ijbiomac.2018.12.231. Epub 2018 Dec 24.
In current study, a polysaccharide (GPP) was successfully extracted from Gynostemma pentaphyllum herb. Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36. Molecular weight and polydispersity (Mw/Mn) of GPP were 4.070 × 10 Da and 1.037, respectively. Primary structure features of GPP were determined to be a polysaccharide by FT-IR and NMR. Fasting blood sugar of diabetic mice decreased from 17.56 mmol/L to 7.42 mmol/L by orally administration of 0.5 mL GPP (1 mg/mL) for 30 days. GPP exhibited a dose-dependent inhibition effect on α-glucosidase activity. Moreover, GPP could inhibit the glucose absorption and affect the protein expression of GLUT2, but not the protein expression of SGLT1. These results indicated GPP could be used as an effective ingredient to prevent and cure diabetes.
在本研究中，成功地从绞股蓝草中提取了一种多糖（GPP）。GPP 的单糖组成为鼠李糖、阿拉伯糖、半乳糖、葡萄糖、木糖、甘露糖、半乳糖醛酸和葡萄糖醛酸，摩尔比为 4.11:7.34:13.31:20.99:1.07:0.91:4.75:0.36。GPP 的分子量和多分散性（Mw/Mn）分别为 4.070×10 Da 和 1.037。GPP 的红外光谱和核磁共振谱表明其一级结构特征为多糖。通过灌胃 30 天 0.5 mL GPP（1 mg/mL），糖尿病小鼠的空腹血糖从 17.56 mmol/L 降至 7.42 mmol/L。GPP 对α-葡萄糖苷酶活性表现出剂量依赖性抑制作用。此外，GPP 可抑制葡萄糖吸收并影响 GLUT2 的蛋白表达，但不影响 SGLT1 的蛋白表达。这些结果表明 GPP 可作为有效成分用于预防和治疗糖尿病。
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