Zhang Yun, Wen Xuan, Xu Neng, Fu Hongyan, Lv Ge, Yu Wenjie, Wei Lina, Zhao Lin
College of Food Engineering, Heilongjiang East University, Harbin 150066, China.
Quality & Safety Institute of Agricultural Products, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China.
Molecules. 2025 Feb 18;30(4):949. doi: 10.3390/molecules30040949.
L. fruit polysaccharides can reduce blood sugar, regulate blood lipids, and improve intestinal flora structure. However, the specific polysaccharide components exerting these effects are unclear. In this study, we extracted, separated, purified, and characterized the polysaccharides Phy-1a, Phy-1b, and Phy-1c. Ion chromatography showed that Phy-1b was mainly composed of rhamnose, arabinose, galactose, glucose, and xylose at a molar ratio of 3.0:19.8:47.5:20.9:8.8, and Phy-1c was composed of rhamnose, arabinose, galactose, glucose, xylose, mannose, ribose Galactosamine hydrochloride and Glucosamine hydrochloride at a molar ratio of 10.4:7.9:22.8:30.5:4.6:4.4:19.4:3.9:5.8. Neither of these polysaccharides contained uronic acid, indicating their neutral property. Methylation analysis and nuclear magnetic resonance spectroscopy showed that Phy-1b was mainly composed of terminal sugars (1-Araf); 1,5-Araf; 1,4-Xylp; 1-Glcp; 2,4-Rhap; 1,3-Glcp; 1,4-Galp; 1,4-Glcp; 1,3-Galp; 1,6-Glcp; 1,3,6-Glcp; and 1,4,6-Galp at a molar ratio of 5.2:7.1:7.8:13.7:6.3:11.2:7.0:16.3:7.4:6.0:6.8:5.3, with the main chain being →2)-α-L-Rhap-(1→4)-β-d-Galp-(1→4)-β-d-Galp-(1→[3)-β-d-Glcp-(1]2→3)-β-d-Glcp-(1→[4)-β-d-Glcp-(1]2→ and the branched chains being β-L-Araf-(1→5)-β-L-Araf-(1→, β-d-Glcp-(1→4)-β-d-Xylp-(1→ 3)-β-d-Galp-(1→, and β-d-Glcp-(1→6)-β-d-Glcp-(1→. The three fragments, respectively, pass through the O-4 key of →2,4)-α-l-Rhap-(1→, O-6 key of →4,6)-β-d-Galp-(1→, and O-6 of →3,6)-β-d-Glcp-(1→ connected to the main chain. These results provide a reference for enhancing the utilization value of resources to promote its high-value and efficient processing.
L.果多糖具有降血糖、调节血脂和改善肠道菌群结构的作用。然而，发挥这些作用的具体多糖成分尚不清楚。在本研究中，我们对多糖Phy-1a、Phy-1b和Phy-1c进行了提取、分离、纯化和表征。离子色谱显示，Phy-1b主要由鼠李糖、阿拉伯糖、半乳糖、葡萄糖和木糖组成，摩尔比为3.0:19.8:47.5:20.9:8.8，Phy-1c由鼠李糖、阿拉伯糖、半乳糖、葡萄糖、木糖、甘露糖、核糖、盐酸半乳糖胺和盐酸葡萄糖胺组成，摩尔比为10.4:7.9:22.8:30.5:4.6:4.4:19.4:3.9:5.8。这两种多糖均不含糖醛酸，表明它们具有中性性质。甲基化分析和核磁共振光谱表明，Phy-1b主要由末端糖（1-Araf）；1,5-Araf；1,4-Xylp；1-Glcp；2,4-Rhap；1,3-Glcp；1,4-Galp；1,4-Glcp；1,3-Galp；1,6-Glcp；1,3,6-Glcp；和1,4,6-Galp组成，摩尔比为5.2:7.1:7.8:13.7:6.3:11.2:7.0:16.3:7.4:6.0:6.8:5.3，主链为→2)-α-L-Rhap-(1→4)-β-d-Galp-(1→4)-β-d-Galp-(1→[3)-β-d-Glcp-(1]2→3)-β-d-Glcp-(1→[4)-β-d-Glcp-(1]2→，支链为β-L-Araf-(1→5)-β-L-Araf-(1→，β-d-Glcp-(1→4)-β-d-Xylp-(1→ 3)-β-d-Galp-(1→，和β-d-Glcp-(1→6)-β-d-Glcp-(1→。这三个片段分别通过→2,4)-α-l-Rhap-(1→的O-4键、→4,6)-β-d-Galp-(1→的O-6键和→3,6)-β-d-Glcp-(1→的O-6键连接到主链上。这些结果为提高资源利用价值以促进其高值高效加工提供了参考。
