Leméteil Denis, Gargala Gilles, Razakandrainibe Romy, Ballet Jean Jacques, Favennec Loic, Costa Damien
Department of Parasitology, Rouen University Hospital, Rouen, France.
Department of Medicine and Pharmacy, Normandie University, and Department of Parasitology, Rouen University Hospital, Rouen, France.
Lab Med. 2019 Jul 16;50(3):243-248. doi: 10.1093/labmed/lmy072.
Effective stool concentration is essential in microscopically based diagnosis of human intestinal parasite infections.
To compare the performances of 4 concentration commercial kits and 1 homemade procedure in 96 clinical stool specimens that tested positive for the detection of 9 helminth and 8 protozoa parasites.
The presence or absence of parasite forms was microscopically determined under conditions of standard practice. Also, we established the accuracies, concentration factors, and extraction yields.
No difference was observed between procedures for preconcentration specimens that tested positive. However, for preconcentration specimens that tested negative, we discovered that the homemade procedure was the most effective, and 2 of the 4 commercial kits were discovered to be satisfactory for routine applications.
For all parasites, procedures with biphasic solvents exhibited higher performances than organic solvent-free procedures. For the first time, the effectiveness of commercial concentration kits has been evaluated on several common stool parasites, and the results suggest that improvement of commercial procedures is possible.
有效的粪便浓缩对于基于显微镜的人体肠道寄生虫感染诊断至关重要。
比较4种商用浓缩试剂盒和1种自制方法对96份临床粪便标本的检测性能，这些标本经检测对9种蠕虫和8种原生动物寄生虫呈阳性。
在标准操作条件下通过显微镜确定寄生虫形态的有无。此外，我们还确定了准确率、浓缩系数和提取率。
对检测呈阳性的预浓缩标本，各方法之间未观察到差异。然而，对于检测呈阴性的预浓缩标本，我们发现自制方法最有效，并且4种商用试剂盒中有2种被发现可满足常规应用。
对于所有寄生虫，使用双相溶剂的方法比无有机溶剂的方法表现出更高的性能。首次对几种常见粪便寄生虫评估了商用浓缩试剂盒的有效性，结果表明改进商用方法是可能的。
