8-甲氧基补骨脂素的脉冲辐解研究

Pulse-radiolysis studies of 8-methoxypsoralen.

作者信息

Redpath J L, Ihara J, Patterson L K

出版信息

Int J Radiat Biol Relat Stud Phys Chem Med. 1978 Apr;33(4):309-15. doi: 10.1080/09553007814550221.

Abstract

Both eaq- and .OH have been found to react with 8-methoxypsoralen (8-MOP), giving rate-constants of 1.1 X 10(10) M-1 s-1. Transient spectra of products from the reactions of eaq-, .OH with 8-MOP have been characterized. Rate-constants for the oxidation by 8-MOP of reduced and oxidized DNA bases have also been measured and found to lie in the range 3-6 X 10(9) M-1 s-1. Oxidation of reduced bases occurs by electron transfer with 100 per cent efficiency in all cases. However, for oxidized bases, only approximately 25 per cent of the intermediate yield produced by OH attack undergoes electron transfer; the balance of the oxidized base appears to form adducts with 8-MOP.

摘要

已发现水合电子（eaq-）和羟基自由基（·OH）均能与8-甲氧基补骨脂素（8-MOP）发生反应，反应速率常数为1.1×10¹⁰ M⁻¹ s⁻¹。已对水合电子、羟基自由基与8-甲氧基补骨脂素反应产物的瞬态光谱进行了表征。还测定了8-甲氧基补骨脂素对还原型和氧化型DNA碱基的氧化反应速率常数，发现其范围为3 - 6×10⁹ M⁻¹ s⁻¹。在所有情况下，还原型碱基的氧化均通过电子转移进行，效率为100%。然而，对于氧化型碱基，羟基自由基攻击产生的中间产物只有约25%通过电子转移；氧化型碱基的其余部分似乎与8-甲氧基补骨脂素形成加合物。