Kato Yukinari, Yamada Shinji, Itai Shunsuke, Kobayashi Atsushi, Konnai Satoru, Kaneko Mika K
1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine , Sendai, Miyagi, Japan .
2 New Industry Creation Hatchery Center, Tohoku University , Sendai, Miyagi, Japan .
Monoclon Antib Immunodiagn Immunother. 2018 Dec;37(6):272-274. doi: 10.1089/mab.2018.0044.
Podoplanin (PDPN) is expressed in lymphatic endothelial cells, where it induces platelet aggregation through C-type lectin-like receptor-2 (CLEC-2). This protein has been characterized for a number of animal species using specific anti-PDPN monoclonal antibodies (mAbs). We recently established the mAb against horse PDPN (horPDPN) named PMab-219. Therefore, in this study, we investigated whether PMab-219 can detect lymphatic endothelial cells in horse tissues. Immunohistochemical analysis demonstrated that PMab-219 strongly stained lymphatic endothelial cells in horse colon tissues, indicating that it will be useful for investigating the function of horPDPN in these cells.
血小板结合蛋白（PDPN）在淋巴管内皮细胞中表达，在那里它通过C型凝集素样受体2（CLEC-2）诱导血小板聚集。使用特异性抗PDPN单克隆抗体（mAb）已对许多动物物种的这种蛋白质进行了表征。我们最近制备了针对马PDPN（horPDPN）的单克隆抗体PMab-219。因此，在本研究中，我们研究了PMab-219是否能检测马组织中的淋巴管内皮细胞。免疫组织化学分析表明，PMab-219对马结肠组织中的淋巴管内皮细胞有强烈染色，表明它将有助于研究horPDPN在这些细胞中的功能。
