Yamada Shinji, Itai Shunsuke, Nakamura Takuro, Takei Junko, Sano Masato, Konnai Satoru, Kobayashi Atsushi, Nakagun Shotaro, Kobayashi Yoshiyasu, Kaneko Mika K, Kato Yukinari
1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
2 Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Monoclon Antib Immunodiagn Immunother. 2019 Jun;38(3):104-107. doi: 10.1089/mab.2019.0014. Epub 2019 Jun 4.
Podoplanin (PDPN)/T1 alpha is known as a specific marker of lymphatic endothelial cells and type I alveolar cells. Sensitive and specific monoclonal antibodies (mAbs) for PDPN are needed for immunohistochemical analyses. Recently, we developed an anticetacean PDPN mAb, PMab-237. Herein, immunohistochemical analyses showed that PMab-237 strongly detected pulmonary type I alveolar cells, renal podocytes, and lymphatic endothelial cells of the harbor porpoise. These findings suggest that PMab-237 may be useful for immunohistochemical analyses for cetacean tissues.
血小板源性生长因子结合蛋白（PDPN）/T1α是淋巴管内皮细胞和I型肺泡细胞的特异性标志物。免疫组织化学分析需要针对PDPN的灵敏且特异的单克隆抗体（mAb）。最近，我们研发了一种抗鲸类PDPN单克隆抗体PMab-237。在此，免疫组织化学分析显示PMab-237能强烈检测出海豚肺I型肺泡细胞、肾足细胞和淋巴管内皮细胞。这些发现表明PMab-237可能对鲸类组织的免疫组织化学分析有用。
