Bashir Khawaja Muhammad Imran, Mohibbullah Md, An Jeong Hyeon, Choi Ji-Yeon, Hong Yong-Ki, Sohn Jae Hak, Kim Jin-Soo, Choi Jae-Suk
Seafood Research Center, IACF, Silla University, Busan, Republic of Korea.
Research Center for Extremophiles and Microbiology, College of Medical and Life Sciences, Silla University, Busan, Republic of Korea.
PeerJ. 2018 Dec 21;6:e6181. doi: 10.7717/peerj.6181. eCollection 2018.
Pacific chub mackerel () is an important fish throughout the world, especially in East Asian countries, including Korea, China, and Japan. Protein hydrolysates from marine sources are commonly used as nutritional supplements, functional ingredients, and flavor enhancers in the food, beverage, and pharmaceutical industries. Antioxidants isolated from fish are relatively easy to prepare, are cost effective, and have no reported side effects. Hence, the present study aimed to investigate the antioxidant activities of mackerel muscle protein hydrolysate (MMPH) prepared using Protamex. The bioactivities of MMPH were investigated in alcoholic fatty liver mice (C57BL/6). Serum alanine aminotransferase and aspartate aminotransferase levels were comparable in test and control mice, whereas serum triglyceride and lipid peroxidation levels significantly ( < 0.05; < 0.001) decreased after administration of MMPH (100-500 mg kg), especially at a concentration of 100 mg kg. A significant ( < 0.05) reduction in xanthine oxidase activity was observed in all groups treated with MMPH (100-500 mg kg), as compared with the control group. Significantly ( < 0.05) higher superoxide dismutase (SOD) activity/protein expression and regulated catalase (CAT) activity/protein expression levels were observed in groups administered MMPH (100-500 mg kg), especially at a concentration of 100 mg kg. These results show that the abundant amino acids of play an important role in the cytosol of the liver cells by directly participating in the expression of xanthine oxidase and the detoxifying SOD and CAT proteins, thereby enhancing antioxidant ability and ultimately, inhibiting lipid peroxidation. This study demonstrated that muscle protein hydrolysate from has strong antioxidant activities.
太平洋竹荚鱼（）是一种在全球尤其是在东亚国家（包括韩国、中国和日本）都很重要的鱼类。海洋来源的蛋白质水解物通常在食品、饮料和制药行业用作营养补充剂、功能成分和风味增强剂。从鱼类中分离出的抗氧化剂相对容易制备，具有成本效益，且无副作用报告。因此，本研究旨在调查使用Protamex制备的竹荚鱼肌肉蛋白水解物（MMPH）的抗氧化活性。在酒精性脂肪肝小鼠（C57BL/6）中研究了MMPH的生物活性。试验组和对照组小鼠的血清丙氨酸氨基转移酶和天冬氨酸氨基转移酶水平相当，而在给予MMPH（100 - 500毫克/千克）后，血清甘油三酯和脂质过氧化水平显著降低（<0.05；<0.001），尤其是在浓度为100毫克/千克时。与对照组相比，在所有用MMPH（100 - 500毫克/千克）处理的组中观察到黄嘌呤氧化酶活性显著降低（<0.05）。在用MMPH（100 - 500毫克/千克）给药的组中，尤其是在浓度为100毫克/千克时，观察到超氧化物歧化酶（SOD）活性/蛋白质表达和调节的过氧化氢酶（CAT）活性/蛋白质表达水平显著更高（<0.05）。这些结果表明，的丰富氨基酸通过直接参与黄嘌呤氧化酶以及解毒的SOD和CAT蛋白的表达，在肝细胞的细胞质中发挥重要作用，从而增强抗氧化能力并最终抑制脂质过氧化。本研究表明，来自的肌肉蛋白水解物具有很强的抗氧化活性。
