Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.
Mar Drugs. 2018 Dec 28;17(1):12. doi: 10.3390/md17010012.
, an IclR-like regulator, was selected as the target gene to activate new secondary metabolites in the marine-derived OUC6819. Inactivation of the gene in . OUC6819 led to the accumulation of a new fatty acid amide (), with an unusual 3-amino-butyl acid as the amine component. Moreover, its parent fatty acid () was also discovered both in the wild-type and mutant strains, which was for the first time isolated from a natural source. The structures of compounds and were elucidated by combination of LC-MS and NMR spectroscopic analyses. This study demonstrated that the homologs might serve as a target for new compound activation in strains.
, 一种 IclR 样调控蛋白,被选为海洋来源的 OUC6819 中激活新次生代谢物的靶基因。在. OUC6819 中, 基因的失活导致一种新的脂肪酸酰胺()的积累,其胺基成分为不寻常的 3-氨基丁酸。此外,其母体脂肪酸()在野生型和 突变株中均被发现,这是首次从天然来源中分离得到。通过 LC-MS 和 NMR 波谱分析的组合,阐明了化合物 和 的结构。本研究表明,同源物可能作为 菌株中新型化合物激活的靶标。