野生型和细胞壁缺陷型 对 Ag⁺ 的生物合成转化为高度稳定的 Ag⁰ 纳米颗粒。
Biosynthetic Conversion of Ag⁺ to highly Stable Ag⁰ Nanoparticles by Wild Type and Cell Wall Deficient Strains of .
机构信息
Nanobiomaterials and Bioprocessing Laboratory (NABLAB), Dan F. Smith Department of Chemical Engineering, Lamar University, Beaumont, TX 77710, USA.
School of Biological Sciences and Engineering, Yachay Tech University, Hacienda San José s/n, San Miguel de Urcuquí 100119, Ecuador.
出版信息
Molecules. 2018 Dec 28;24(1):98. doi: 10.3390/molecules24010098.
In the current study, two different strains of the green, freshwater microalga bioreduced Ag⁺ to silver nanoparticles (AgNPs), which have applications in biosensors, biomaterials, and therapeutic and diagnostic tools. The bioreduction takes place in cell cultures of at ambient temperature and atmospheric pressure, thus eliminating the need for specialized equipment, harmful reducing agents or the generation of toxic byproducts. In addition to the visual changes in the cell culture, the production of AgNPs was confirmed by the characteristic surface plasmon resonance (SPR) band in the range of 415⁻425 nm using UV-Vis spectrophotometry and further evolution of the SPR peaks were studied by comparing the peak intensity at maximum absorbance over time. X-ray diffraction (XRD) determined that the NPs were Ag⁰. Micrographs from transmission electron microscopy (TEM) revealed that 97 ± 2% AgNPs were <10 nm in diameter. Ag⁺ to AgNP conversion was determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES). The AgNPs were stable over time in the cell culture media, acetone, NaCl and reagent alcohol solutions. This was verified by a negligible change in the features of the SPR band after t > 300 days of storage at 4 °C.
在本研究中，两种不同的淡水绿藻菌株将银离子还原为银纳米颗粒（AgNPs），这些颗粒在生物传感器、生物材料以及治疗和诊断工具中具有应用。生物还原在常温常压的细胞培养物中进行，因此无需特殊设备、有害还原剂或产生有毒副产物。除了细胞培养物的可见变化外，还通过使用紫外可见分光光度法在 415-425nm 范围内确认了 AgNPs 的特征表面等离子体共振（SPR）带的产生，并通过比较随时间变化的最大吸收处的峰值强度进一步研究了 SPR 峰的演变。X 射线衍射（XRD）确定 NPs 为 Ag0。透射电子显微镜（TEM）的显微照片显示，97±2%的 AgNPs 直径小于 10nm。通过电感耦合等离子体原子发射光谱法（ICP-AES）确定 Ag+到 AgNP 的转化。AgNPs 在细胞培养介质、丙酮、NaCl 和试剂酒精溶液中随时间稳定。在 4°C 下储存超过 300 天后，SPR 带的特征几乎没有变化，证明了这一点。
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