Department of Chemistry, Faculty of Mathematics and Natural Sciences, Andalas University, Kampus Limau Manis, Padang, West Sumatra, 25163, Indonesia.
Department of Teaching and Education, Raja Ali Haji Maritime University, Tanjungpinang, Riau Archipelago, 29115, Indonesia.
F1000Res. 2022 May 16;10:768. doi: 10.12688/f1000research.54661.2. eCollection 2021.
Most natural plants used in the synthesis of silver nanoparticles are limited to marine plants. To carry out applications, colloidal silver nanoparticles (AgNps) should have appropriate properties such as homogeneous shapes, small and narrow particle size distribution, and long time stability. This study aims to determine the effects of a variable process of AgNps mediated mangrove (RS) leaf extract, and antibacterial activity. : Synthesis of AgNps was carried out by stirring silver nitrate solution with aqueous extract. The characterization of AgNps was carried out using UV-Vis spectrophotometry, X-ray diffraction (XRD), Dynamic Light Scattering (DLS) zetasizer and Transmission Electron Microscopy (TEM). Evaluation of antibacterial activity was carried out on and Reaction conditions such as the concentration of metal ions (0.001 M, 0.005 M, and 0.01 M), extracts (1%, 3%, and 5% v/v), and the reaction time on the size and stability of nanoparticles were also explored. : The UV-Vis spectroscopy showed an absorption of colloidal AgNps in a wavelength range of 403-443 nm. TEM analysis showed that as-synthesized AgNps were spherical in shape with a size range of 5-87 nm. The use of 0.001 M and 0.005 M of Ag resulted in a smaller diameter than the synthesized AgNps, using 0.01 M Ag , in the same extract concentration. The range of zeta potential was -24.9 mV to -27.7 mV. The as-synthesized AgNps were stable for more than one month. The XRD analysis showed four peaks, which were attributed to the face centered cubic crystal structure of metallic silver. The results of the silver nanoparticles synthesis showed good activity on and , with an inhibition zone between 4.1-7.2 mm. The AgNps synthesized with RS leaf extract, which is a reducing agent, showed good potential as an antibacterial component.
大多数用于合成银纳米粒子的天然植物仅限于海洋植物。为了进行应用，胶体银纳米粒子（AgNps）应具有适当的性质，如均匀的形状、小而窄的粒径分布和长时间的稳定性。本研究旨在确定红树林（RS）叶提取物介导的 AgNps 可变过程的影响及其抗菌活性。AgNps 的合成是通过搅拌硝酸银溶液和水提物来进行的。AgNps 的表征采用紫外-可见分光光度法、X 射线衍射（XRD）、动态光散射（DLS）Zetasizer 和透射电子显微镜（TEM）进行。抗菌活性的评价是在 和 上进行的。反应条件如金属离子浓度（0.001 M、0.005 M 和 0.01 M）、提取物浓度（1%、3%和 5%v/v）和反应时间对纳米粒子的尺寸和稳定性也进行了探讨。紫外-可见光谱显示胶体 AgNps 在 403-443nm 波长范围内有吸收。TEM 分析表明，所合成的 AgNps 呈球形，粒径范围为 5-87nm。使用 0.001 M 和 0.005 M 的 Ag 导致直径小于用相同提取物浓度合成的 AgNps，而使用 0.01 M 的 Ag 则导致直径更大。zeta 电位范围为-24.9 mV 至-27.7 mV。所合成的 AgNps 在一个月以上的时间内保持稳定。XRD 分析显示出四个峰，归因于金属银的面心立方晶体结构。银纳米粒子合成的结果表明，对 和 有很好的活性，抑菌圈在 4.1-7.2mm 之间。用 RS 叶提取物作为还原剂合成的 AgNps 显示出作为抗菌成分的良好潜力。
