用环磷酰胺处理的小鼠中，凝乳寡糖比凝乳多糖具有更高的免疫刺激活性。
Curdlan oligosaccharides having higher immunostimulatory activity than curdlan in mice treated with cyclophosphamide.
机构信息
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu, Haidian District, Beijing, 100083, China.
Bioresource Utilization Laboratory, College of Engineering, China Agricultural University, No. 17 Qinghua Donglu, Haidian District, Beijing, 100083, China.
出版信息
Carbohydr Polym. 2019 Mar 1;207:131-142. doi: 10.1016/j.carbpol.2018.10.120. Epub 2018 Nov 22.
This study evaluated the immunostimulatory activity of curdlan oligosaccharides (GOS) in cyclophosphamide (CTX)-induced immunosuppressed mice and in RAW264.7 cells. GOS was able to stimulate the release of nitric oxide (NO), cytokines (IL-1β, IL-6 and TNF-α) and improve the phagocytic rate of peritoneal macrophages and RAW264.7 cells. It further enhanced immunoglobulins (Ig) release (IgG by 50.6%-74.7%, IgA by 31.3%-34.9%, IgM by 28.3%-66.7%), splenic lymphocyte proliferation (by 74.8%-91.3%), nature killer cells cytotoxicity (by 32.0%-49.6%), immunophenotypes of splenic lymphocytes (from 1.7 to 2.4, 2.2 and 2.7) in immunosuppressed mice. Compared with curdlan, higher immunostimulatory activity of GOS was found in CTX-treated mice. Moreover, GOS could activate nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways through toll-like receptor 2 (TLR2) and complement receptor 3 (CR3). These results indicated that GOS may be a favorable candidate of functional food in regulating immune responses.
本研究评估了出芽短梗霉多糖寡糖（GOS）在环磷酰胺（CTX）诱导的免疫抑制小鼠和 RAW264.7 细胞中的免疫刺激活性。GOS 能够刺激一氧化氮（NO）、细胞因子（IL-1β、IL-6 和 TNF-α）的释放，并提高腹腔巨噬细胞和 RAW264.7 细胞的吞噬率。它进一步增强了免疫球蛋白（Ig）的释放（IgG 增加 50.6%-74.7%，IgA 增加 31.3%-34.9%，IgM 增加 28.3%-66.7%）、脾淋巴细胞增殖（增加 74.8%-91.3%）、自然杀伤细胞细胞毒性（增加 32.0%-49.6%）、脾淋巴细胞免疫表型（从 1.7 增加到 2.4、2.2 和 2.7）在免疫抑制小鼠中。与出芽短梗霉相比，GOS 在 CTX 处理的小鼠中具有更高的免疫刺激活性。此外，GOS 可以通过 Toll 样受体 2（TLR2）和补体受体 3（CR3）激活核因子 kappa-轻链增强子的 B 细胞激活（NF-κB）和丝裂原激活蛋白激酶（MAPK）信号通路。这些结果表明，GOS 可能是一种调节免疫反应的功能性食品的候选物。
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