Lei Dan, Ye Yu, Lin Kun, Zhang Fanfan, Huang Dongyan, Li Kai, Yuan Weifeng, Wu Qiong, Ding Zhen, Wang Leyi, Song Deping, Tang Yuxin
Key Laboratory for Animal Health of Jiangxi Province, Nanchang, 330045, Jiangxi, China.
Department of Preventive Veterinary Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China.
Virus Genes. 2019 Apr;55(2):248-252. doi: 10.1007/s11262-018-1624-6. Epub 2019 Jan 2.
Porcine pegiviruses (PPgV) have been first discovered in serum samples from domestic pigs in Germany in 2016 and then in the USA in 2018. To date, there is no documentation with respect to the presence of PPgVs in domestic pigs in China. Herein, we attempted to determine the presence and prevalence of PPgV in China and its genetic characterization. In this study, 469 sera were tested and 34 (7.25%) were positive for PPgV. An ascending trend of the positive rate for PPgV was observed from suckling piglets (1.61%) to nursing piglets (1.85%), finishing pigs (6.56%), and sows (11.34%). The complete genome sequence of a representative strain of PPgV, PPgV_GDCH2017, and the complete E2 gene of 17 PPgV isolates discovered in this study was determined. Sequence analysis indicated that PPgV_GDCH2017 was highly related to other PPgVs with nucleotide and amino acid identities ranging from 87.3 to 97.4% and 94.6-99.3%, respectively, in the complete coding region. Phylogenetic analyses demonstrated that the PPgV_GDCH2017 discovered in this study was closely related to the PPgVs from the USA and clustered in the same genus with pegiviruses from other hosts. The topology of the phylogenetic tree based on the complete E2 gene was consistent with that based on the complete genome of PPgV. Further studies on pathogenicity and pathogenesis of PPgVs are needed.
猪pegivirus（PPgV）于2016年首次在德国家猪的血清样本中被发现，随后于2018年在美国被发现。迄今为止，中国家猪中PPgV存在情况尚无文献记载。在此，我们试图确定PPgV在中国的存在情况、流行率及其基因特征。在本研究中，检测了469份血清，其中34份（7.25%）PPgV呈阳性。观察到PPgV阳性率从哺乳仔猪（1.61%）到保育仔猪（1.85%）、育肥猪（6.56%）和母猪（11.34%）呈上升趋势。确定了一株代表性PPgV毒株PPgV_GDCH2017的全基因组序列以及本研究中发现的17株PPgV分离株的完整E2基因。序列分析表明，PPgV_GDCH2017与其他PPgV高度相关，在完整编码区核苷酸和氨基酸同一性分别为87.3%至97.4%和94.6%至99.3%。系统发育分析表明，本研究中发现的PPgV_GDCH2017与来自美国的PPgV密切相关，并与来自其他宿主的pegivirus聚集在同一属中。基于完整E2基因的系统发育树拓扑结构与基于PPgV全基因组的拓扑结构一致。需要对PPgV的致病性和发病机制进行进一步研究。
