School of Pharmacy, Monash University, Bandar Sunway, Selangor Darul Ehsan, Malaysia.
Institute of Pharmaceutical Sciences (IPS), University of Veterinary & Animal Sciences (UVAS), Lahore, Pakistan.
Nat Prod Res. 2020 Sep;34(18):2602-2606. doi: 10.1080/14786419.2018.1543684. Epub 2019 Jan 2.
In this study, phytochemical composition, antioxidant, enzyme inhibition and cytotoxic activities of methanol and dichloromethane (DCM) extracts of flowers were investigated. Methanol extract was found to have higher total bioactive contents and UHPLC-MS analysis of methanol extract revealed the presence of well-known phenolic and flavonoid compounds. Antioxidant activities were performed by radical scavenging (DPPH and ABTS), reducing power (FRAP and CUPRAC), phosphomolybdenum (TAC) and metal chelating assays. From our result, we observed that methanol extract had many antioxidant compounds. The DCM extract exhibited higher cholinesterases and α-glucosidase enzyme inhibition, while methanol extract showed significant urease inhibition. Both extracts exhibited strong to moderate cytotoxicity against MCF-7, MDA-MB-231, CaSki, DU-145 and SW-480 cancer cells with IC values ranging from 88.49 to 304.7 µg/mL. The findings showed the to possess considerable antioxidant, enzyme inhibition and cytotoxic potentials and therefore has potential to discover novel bioactive molecules.
本研究对 花的甲醇和二氯甲烷（DCM）提取物的植物化学成分、抗氧化、酶抑制和细胞毒性活性进行了研究。发现甲醇提取物具有更高的总生物活性含量，甲醇提取物的 UHPLC-MS 分析显示存在着众所周知的酚类和类黄酮化合物。抗氧化活性通过自由基清除（DPPH 和 ABTS）、还原能力（FRAP 和 CUPRAC）、磷钼酸盐（TAC）和金属螯合测定来进行。根据我们的结果，我们观察到甲醇提取物中含有许多抗氧化化合物。DCM 提取物对胆碱酯酶和α-葡萄糖苷酶表现出较高的抑制作用，而甲醇提取物对脲酶表现出显著的抑制作用。两种提取物对 MCF-7、MDA-MB-231、CaSki、DU-145 和 SW-480 癌细胞均具有较强至中度的细胞毒性，IC 值范围为 88.49 至 304.7 µg/mL。研究结果表明 具有相当大的抗氧化、酶抑制和细胞毒性潜力，因此有可能发现新的生物活性分子。
