Zengin Gokhan, Mahomoodally Mohamad Fawzi, Sinan Kouadio Ibrahime, Picot-Allain Marie Carene Nancy, Yildiztugay Evren, Cziáky Zoltán, Jekő József, Saleem Hammad, Ahemad Nafees
Department of Biology, Science Faculty, Selçuk University, Campus, Konya, Turkey.
Institute of Research and Development, Duy Tan University, Da Nang 550000, VietNam; Department of Health Sciences, Faculty of Science, University of Mauritius, 230 Réduit, Mauritius.
Food Res Int. 2020 Jul;133:109129. doi: 10.1016/j.foodres.2020.109129. Epub 2020 Feb 28.
The Crocus and Cyclamen genus have been reported to possess diverse biological properties. In the present investigation, two geophytes from these genus, namely Crocus pallasi and Cyclamen cilicium have been studied. The in vitro antioxidant, enzyme inhibitory, and cytotoxic effects of the methanol extracts of Crocus pallasii and Cyclamen cilicium aerial and underground parts were investigated. Antioxidant abilities of the extracts were investigated via different antioxidant assays (metal chelating, radical quenching (ABTS and DPPH), reducing power (CUPRAC and FRAP) and phosphomolybdenum). Cholinesterases, amylase, tyrosinase, and glucosidase were used as target enzymes for detecting enzyme inhibitory abilities of the samples. Regarding the cytotoxic abilities, breast cancer cell lines (MDA-MB 231 and MCF-7) and prostate cancer cell lines (DU-145) were used. The flowers extracts of Crocus pallasii and C. cilicium possessed the highest flavonoid content. The highest phenolic content was recorded from C. cilicium root extract (47.62 mg gallic acid equivalent/g extract). Cyclamen cilicium root extract showed significantly (p < 0.05) high radical scavenging (94.28 and 139.60 mg trolox equivalent [TE]/g extract, against DPPH and ABTS radicals, respectively) and reducing potential (173.30 and 109.53 mg TE/g extract, against CUPRAC and FRAP, respectively). The best acetylcholinesterase, glucosidase and tyrosinase inhibition was observed in C. cilicium root (4.46 mg GALAE/g; 15.75 mmol ACAE/g; 136.99 mg KAE/g, respectively). Methanolic extracts of C. pallasii and C. cilicium showed toxicity against breast cancer cell lines. In light of the above findings, C. cilicium might be considered as an interesting candidate in the development of anti-cancer agent coupled with antioxidant properties.
据报道，番红花属和仙客来属植物具有多种生物学特性。在本研究中，对这两个属的两种地下芽植物，即帕氏番红花（Crocus pallasi）和西里西亚仙客来（Cyclamen cilicium）进行了研究。研究了帕氏番红花和西里西亚仙客来地上和地下部分甲醇提取物的体外抗氧化、酶抑制和细胞毒性作用。通过不同的抗氧化试验（金属螯合、自由基淬灭（ABTS和DPPH）、还原能力（CUPRAC和FRAP）和磷钼酸法）研究了提取物的抗氧化能力。使用胆碱酯酶、淀粉酶、酪氨酸酶和糖苷酶作为靶酶来检测样品的酶抑制能力。关于细胞毒性能力，使用了乳腺癌细胞系（MDA-MB 231和MCF-7）和前列腺癌细胞系（DU-145）。帕氏番红花和西里西亚仙客来的花提取物黄酮含量最高。西里西亚仙客来根提取物的酚类含量最高（47.62毫克没食子酸当量/克提取物）。西里西亚仙客来根提取物表现出显著（p < 0.05）的高自由基清除能力（分别针对DPPH和ABTS自由基，为94.28和139.60毫克 Trolox当量[TE]/克提取物）和还原能力（分别针对CUPRAC和FRAP，为173.30和109.53毫克TE/克提取物）。在西里西亚仙客来根中观察到最佳的乙酰胆碱酯酶、糖苷酶和酪氨酸酶抑制效果（分别为4.46毫克GALAE/克；15.75毫摩尔ACAE/克；136.99毫克KAE/克）。帕氏番红花和西里西亚仙客来的甲醇提取物对乳腺癌细胞系显示出毒性。根据上述发现，西里西亚仙客来可能被认为是开发具有抗氧化特性的抗癌药物的一个有吸引力的候选植物。
