Mohamed Wan Ahmad Syazani, Ismail Noor Zafirah, Muhamad Musthahimah, Omar Eshaifol Azam, Abdul Samad Nozlena, Ooi Jer Ping, Mohamad Sharlina
Centre for Coordination of Clinical Research Network (CCRN), Block B4, Institute for Clinical Research (ICR), National Institutes of Health (NIH), Jalan Setia Murni U13/52, Seksyen U13, Setia Alam, 40170 Shah Alam, Selangor, Malaysia.
Centre for Clinical Trial (CCT), Block B4, Institute for Clinical Research (ICR), National Institutes of Health (NIH), Jalan Setia Murni U13/52, Seksyen U13, Setia Alam, 40170 Shah Alam, Selangor, Malaysia.
Saudi J Biol Sci. 2022 Oct;29(10):103403. doi: 10.1016/j.sjbs.2022.103403. Epub 2022 Aug 5.
Propolis is known to exhibit various phytochemical compounds that aid in several biological activities. The current study investigates the phytochemical compounds of ethanolic extract of propolis of (EEP) using Q-TOF LC-MS, its antioxidant properties using DPPH and ABTS radical scavenging assays, total phenolic (TPC) and flavonoid content (TFC), using Folin-Ciocalteu and Aluminium Chloride method, respectively, as well as proapoptotic effects, based on the selected IC of the cytotoxic study conducted for EEP using annexin V-FITC assay. Terpene and polyphenol were among of 17 identified compounds. The EC of EEP for DPPH and ABTS was 1.78 mg/mL and 1.68 mg/mL, while the EEP exhibited TPC and TFC values of 31.99 mgGAE/g and 66.4 mgQCE/g, respectively in which the parameters were strongly correlated. The IC of EEP effectively induces apoptosis in MCF7 cells. In conclusion, EEP possessed important phytochemical compounds that work excellently as antioxidants and anticancer agents.
已知蜂胶含有多种植物化学化合物，有助于多种生物活性。本研究使用Q-TOF LC-MS研究了蜂胶乙醇提取物（EEP）的植物化学化合物，通过DPPH和ABTS自由基清除试验研究了其抗氧化性能，分别使用福林-西奥尔特试剂法和氯化铝法测定了总酚含量（TPC）和类黄酮含量（TFC），并基于使用膜联蛋白V-FITC测定法对EEP进行的细胞毒性研究中选定的IC50值研究了促凋亡作用。萜烯和多酚是17种已鉴定化合物中的一部分。EEP对DPPH和ABTS的EC50分别为1.78 mg/mL和1.68 mg/mL，而EEP的TPC和TFC值分别为31.99 mg GAE/g和66.4 mg QCE/g，其中这些参数呈强相关。EEP的IC50有效诱导MCF7细胞凋亡。总之，EEP拥有重要的植物化学化合物，作为抗氧化剂和抗癌剂表现出色。
