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利用转录组学鉴定武进猪和杜洛克猪造血差异的候选基因

Use of Transcriptomics to Identify Candidate Genes for Hematopoietic Differences Between Wujin and Duroc Pigs.

作者信息

Ji Peng, Wang Ping, Li Qihua, Gao Lin, Xu Yan, Pan Hongbin, Zhang Chunyong, Li Jintao, Yao Jun, An Qingcong

机构信息

Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China.

Yunnan Tropical and Subtropical Animal Virus Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming 650224, China.

出版信息

Animals (Basel). 2024 Dec 4;14(23):3507. doi: 10.3390/ani14233507.

DOI:10.3390/ani14233507
PMID:39682471
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11639938/
Abstract

Hematopoiesis is a complex physiological process that ensures renewal of blood cells to maintain normal blood circulation and immune function. Wujin pigs exhibit distinct characteristics such as tender meat, high fat storage, strong resistance to roughage, robust disease resistance, and oxidation resistance. Therefore, using Wujin pigs as models may offer valuable insights for hematopoietic-related studies. In this study, twelve healthy 35-day-old piglets, including six Wujin and six Duroc piglets of similar weight, were selected from each of the Wujin and Duroc pig groups and housed in single cages. After 30 days of feeding, blood and bone marrow samples were collected. Routine blood indices and hematopoietic-related serum biochemical indexes of Wujin and Duroc pigs were determined, and bone marrow gene expression levels were analyzed using transcriptomics. (1) Hemoglobin (Hb) and Mean Corpuscular Hemoglobin Concentration (MCHC) levels in Wujin pigs were significantly higher than in Duroc pigs ( < 0.05), and platelet counts and serum Hb levels in Wujin pigs were significantly lower than in Duroc pigs ( < 0.05). (2) A total of 312 significantly differentially expressed genes were identified between the pigs. Their functions were mainly related to blood systems, inflammation, and oxidation. Six differentially expressed genes may be related to hematopoietic function. (3) By combining the differential genes screened through sequencing with Weighted Gene Co-expression Network Analysis results, 16 hematopoietic function differential genes were obtained, mainly focusing on immunity, inflammation, and induction of apoptosis functions. Differences were present in the immune and inflammatory responses between Wujin pigs and Duroc pigs, suggesting that differences in hematopoietic function between the two breeds were related to antioxidant capacity and disease resistance.

摘要

造血是一个复杂的生理过程，可确保血细胞更新以维持正常血液循环和免疫功能。武进猪具有肉质鲜嫩、脂肪储存量高、耐粗饲能力强、抗病力强和抗氧化能力强等显著特点。因此，以武进猪为模型可能为造血相关研究提供有价值的见解。在本研究中，从武进猪和杜洛克猪组中各选出12头健康的35日龄仔猪，包括6头体重相近的武进仔猪和6头杜洛克仔猪，并单笼饲养。饲养30天后，采集血液和骨髓样本。测定了武进猪和杜洛克猪的常规血液指标和造血相关血清生化指标，并使用转录组学分析了骨髓基因表达水平。（1）武进猪的血红蛋白（Hb）和平均红细胞血红蛋白浓度（MCHC）水平显著高于杜洛克猪（<0.05），武进猪的血小板计数和血清Hb水平显著低于杜洛克猪（<0.05）。（2）在这些猪之间共鉴定出312个显著差异表达基因。它们的功能主要与血液系统、炎症和氧化有关。六个差异表达基因可能与造血功能有关。（3）通过将测序筛选出的差异基因与加权基因共表达网络分析结果相结合，获得了16个造血功能差异基因，主要集中在免疫、炎症和诱导细胞凋亡功能上。武进猪和杜洛克猪在免疫和炎症反应方面存在差异，这表明两个品种在造血功能上的差异与抗氧化能力和抗病力有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a7e/11639938/62e155f0ba6c/animals-14-03507-g001.jpg
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