Lee Jaeyeon, Song Myeongjin, Kim Jongseong, Park Yongdoo
Department of Biomedical Engineering, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul, 02841 Republic of Korea.
Tissue Eng Regen Med. 2018 Jul 5;15(4):493-502. doi: 10.1007/s13770-018-0134-x. eCollection 2018 Aug.
The interplay between neurogenesis and angiogenesis is crucial during the development mediated by neuro-angiogenic morphogens. In particular, the angiogenic activity of neuropeptides and their role in tissue regeneration have long been investigated for better understanding of their biological mechanisms and further applications. However, there have been few studies for direct comparison of angiogenic activities of neuropeptides for and models. In this study, we report that direct comparison of the angiogenic activities of neuropeptide Y, secretoneurin, and substance P (SP) immobilized on hydrogels in and experiments.
A hyaluronic acid-based hydrogel is prepared by utilizing acrylated hyaluronic acid and thiolated peptides as a crosslinker and angiogenic factors, respectively. Angiogenic activities of three neuropeptides are evaluated not only by angiogenic and gene expression assays, but also by an chronic myocardial infarction model.
The comparison of angiogenic activities of three peptides demonstrates that the SP-immobilized hydrogel shows a higher degree of cell network formation and angiogenic-specific genes than those of the other peptides and the control case. In addition, a three-dimensional angiogenic assay illustrates that more sprouting is observable in the SP group. Evaluation of regenerative activity in the chronic myocardial infarction model reveals that all three peptide-immobilized hydrogels induce increased cardiac function as well as structural regeneration. Among all the cases, the SP group provided the highest regenerative activity both and .
In our comparison study, the SP-immobilized hydrogel shows the highest angiogenic activity and tissue regeneration among the test groups. This result suggests that nerve regeneration factors help angiogenesis in damaged tissues, which also highlights the importance of the neuro-angiogenic peptides as an element of tissue regeneration.
在由神经血管生成形态发生素介导的发育过程中，神经发生与血管生成之间的相互作用至关重要。特别是，长期以来一直在研究神经肽的血管生成活性及其在组织再生中的作用，以更好地理解其生物学机制并进一步应用。然而，很少有研究直接比较神经肽在[具体模型1]和[具体模型2]中的血管生成活性。在本研究中，我们报告了在[具体模型1]和[具体模型2]实验中对固定在水凝胶上的神经肽Y、分泌素和P物质（SP）的血管生成活性进行直接比较的情况。
分别利用丙烯酸化透明质酸和硫醇化肽作为交联剂和血管生成因子，制备了基于透明质酸的水凝胶。不仅通过[具体模型1]血管生成和基因表达测定，还通过[具体模型2]慢性心肌梗死模型评估三种神经肽的血管生成活性。
三种肽的[具体模型1]血管生成活性比较表明，固定有SP的水凝胶比其他肽和对照情况显示出更高程度的细胞网络形成和血管生成特异性基因。此外，三维血管生成测定表明，在SP组中可观察到更多的芽生。慢性心肌梗死模型中的再生活性评估表明，所有三种固定有肽的水凝胶均诱导心脏功能增加以及结构再生。在所有情况中，SP组在[具体模型1]和[具体模型2]中均提供了最高的再生活性。
在我们的比较研究中，固定有SP的水凝胶在测试组中显示出最高的血管生成活性和组织再生能力。这一结果表明神经再生因子有助于受损组织中的血管生成，这也突出了神经血管生成肽作为组织再生要素的重要性。
