1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
2Marine Drugs Research Center, State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacy, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, PR China.
Int J Syst Evol Microbiol. 2019 Mar;69(3):715-720. doi: 10.1099/ijsem.0.003209. Epub 2019 Jan 3.
A novel marine actinomycete, designated LHW63014, isolated from a marine sponge, Craniella species, collected in the South China Sea, was examined using a polyphasic approach. The 16S rRNA gene sequence of strain LHW63014 showed highest similarities to Jishengella endophytica 202201 (98.9 %), Micromonospora olivasterospora DSM 43868 (98.7 %), Micromonospora maris AB-18-032 (98.6 %) and Micromonospora gifhornensis DSM 44337 (98.6 %). Phylogenetic analyses of the family Micromonosporaceae based on the 16S rRNA and gyrB gene sequences indicated that strain LHW63014 and J. endophytica 202201 located within the genus Micromonospora. Morphological and chemotaxonomic characteristics confirmed their affiliation to the genus Micromonospora. Based on phenotypic characteristics, phylogenetic data and digital DNA-DNA hybridization results, strain LHW63014 could be distinguished from its closest taxa, representing a novel species of the genus Micromonospora, for which the name Micromonospora craniellae sp. nov., is proposed, with the type strain LHW63014 (=DSM 106193=CCTCC AA 2018012). It is also proposed that J. endophytica be transferred to genus Micromonospora as Micromonospora endophytica comb. nov. (type strain 202201 =CGMCC 4.5597=DSM 45430).
一株新型海洋放线菌，命名为 LHW63014，从南海采集的海绵 Craniella 属中分离得到，采用多相分类法进行了研究。菌株 LHW63014 的 16S rRNA 基因序列与 Jishengella endophytica 202201（98.9%）、Micromonospora olivasterospora DSM 43868（98.7%）、Micromonospora maris AB-18-032（98.6%）和 Micromonospora gifhornensis DSM 44337（98.6%）最为相似。基于 16S rRNA 和 gyrB 基因序列的 Micromonosporaceae 科系统发育分析表明，菌株 LHW63014 和 J. endophytica 202201 位于 Micromonospora 属内。形态学和化学分类特征证实了它们与 Micromonospora 属的隶属关系。基于表型特征、系统发育数据和数字 DNA-DNA 杂交结果，菌株 LHW63014 可与最接近的分类群区分开来，代表了 Micromonospora 属的一个新种，命名为 Micromonospora craniellae sp. nov.，其模式菌株为 LHW63014（=DSM 106193=CCTCC AA 2018012）。还提议将 J. endophytica 转移到 Micromonospora 属中，作为 Micromonospora endophytica comb. nov.（模式菌株 202201=CGMCC 4.5597=DSM 45430）。
