Division of Applied Material Science, Department of Engineering Sciences, The Ångström Laboratory, Uppsala University, Sweden.
Division of Applied Material Science, Department of Engineering Sciences, The Ångström Laboratory, Uppsala University, Sweden.
Mater Sci Eng C Mater Biol Appl. 2019 Mar;96:272-279. doi: 10.1016/j.msec.2018.11.021. Epub 2018 Nov 20.
Bacterial infections associated with metal implants are severe problems affecting a considerable amount of people with dental or orthopedic implants. This study aims to examine the antibacterial effect of a Titanium-peroxy gel layer on the modified surface of commercially pure titanium grade 2. Variations in a multi-step surface modification procedure were tested to determine the best combination that provided an antibacterial effect while enhancing bioactivity without compromising biocompatibility. Soaking the surfaces in 30 wt% hydrogen peroxide held at 80 °C provided antibacterial activity while subsequent surface treatments in concentrated sodium and calcium hydroxide solutions were preformed to enhance bioactivity. Staphylococcus epidermidis was used to determine the antibacterial effect through both direct contact and biofilm inhibition tests while human dermal fibroblast cells and MC3T3 pre osteoblast cells were utilized to test biocompatibility. The greatest antibacterial effect was observed with only hydrogen peroxide treatment, but the resulting surface was neither bioactive nor biocompatible. It was found that subsequent surface treatments with sodium hydroxide followed by calcium hydroxide provided a bioactive surface that was also biocompatible. Additionally, a final treatment with autoclaving showed positive effects with regards to enhanced bioactivity. This multi-step surface modification procedure offers a promising, non-antibiotic, solution for combatting infections associated with biomedical implants.
金属植入物相关的细菌感染是影响大量牙科或骨科植入物患者的严重问题。本研究旨在研究钛过氧凝胶层在商业纯钛 2 级改良表面上的抗菌效果。测试了多步表面改性程序的变化，以确定最佳组合，在不影响生物相容性的情况下提供抗菌效果同时增强生物活性。将表面浸泡在 80°C 的 30wt%过氧化氢中可提供抗菌活性，随后在浓氢氧化钠和氢氧化钙溶液中进行表面处理以增强生物活性。表皮葡萄球菌用于通过直接接触和生物膜抑制试验来确定抗菌效果，同时用人真皮成纤维细胞和 MC3T3 前成骨细胞来测试生物相容性。仅用过氧化氢处理观察到最大的抗菌效果，但所得表面既无生物活性也无生物相容性。结果发现，随后用氢氧化钠处理再用氢氧化钙处理提供了具有生物活性且生物相容的表面。此外，最后用高压灭菌处理显示出增强生物活性的积极效果。这种多步表面改性程序为对抗与生物医学植入物相关的感染提供了一种有前途的非抗生素解决方案。
