Advanced Institute for Science and Technology (AIST), Hanoi University of Science and Technology, Viet Nam; Hung Yen University of Technology and Education, Viet Nam.
Advanced Institute for Science and Technology (AIST), Hanoi University of Science and Technology, Viet Nam.
Mater Sci Eng C Mater Biol Appl. 2019 Mar;96:790-797. doi: 10.1016/j.msec.2018.11.059. Epub 2018 Nov 28.
A core-shell cerium oxide nanorod@polypyrrole (CeO-NR@Ppy) nanocomposite-based electrochemical DNA biosensor was studied for Salmonella detection. The core-shell CeO-NR@Ppy nanocomposite was prepared by in situ chemical oxidative polymerization of pyrrole monomer on CeO-NRs, which provided a suitable platform for electrochemical DNA biosensor fabrication. The immobilization of ss-DNA sequences onto nanocomposite-coated microelectrode was performed via covalent attachment method. DNA biosensor electrochemical responses were studied by cyclic voltammetry and electrochemical impedance spectroscopy with [Fe (CN)] as redox probe. Under optimal conditions, DNA biosensor response showed good linearity in the range of 0.01-0.4 nM with sensitivity of 593.7 Ω·nM·cm. The low limit of detection and limit of quantification for the DNA biosensor were 0.084 and 0.28 nM, respectively. The proposed DNA biosensor also showed good results when used in detecting actual Salmonella samples.
基于核壳氧化铈纳米棒@聚吡咯（CeO-NR@Ppy）纳米复合材料的电化学 DNA 生物传感器被用于检测沙门氏菌。CeO-NR@Ppy 核壳纳米复合材料通过 CeO-NRs 上吡咯单体的原位化学氧化聚合制备，为电化学 DNA 生物传感器的制备提供了合适的平台。ss-DNA 序列通过共价附着方法固定在纳米复合材料涂覆的微电极上。采用循环伏安法和电化学阻抗谱法，以[Fe（CN）]作为氧化还原探针研究 DNA 生物传感器的电化学响应。在最佳条件下，DNA 生物传感器的响应在 0.01-0.4 nM 范围内表现出良好的线性，灵敏度为 593.7 Ω·nM·cm。该 DNA 生物传感器的检测限和定量限分别为 0.084 和 0.28 nM。该 DNA 生物传感器在检测实际沙门氏菌样品时也取得了良好的效果。
