Faramarzian Azimi Maragheh Behnaz, Fatourachi Parisa, Mohammadi Seyede Momeneh, Valipour Behnaz, Behtari Meysam, Dehnad Alireza, Nozad Charoudeh Hojjatollah
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Tabriz Higher Education Institute of Rab-Rashid, Department of Biological Sciences, Tabriz, Iran.
Adv Pharm Bull. 2018 Nov;8(4):675-682. doi: 10.15171/apb.2018.076. Epub 2018 Nov 29.
Streptomyces sp., a dominant genus in Actinomycetes, is the source of a wide variety of secondary metabolites. Microbial metabolites can be utilized as novel anticancer agents; with fewer side effects. The present article illustrated the anti-carcinogenic effect of the ether extracted organic metabolites derived from Streptomyces bacteria on SW480 colon cancer cell line. MTT assay was performed in order to investigate the cytotoxicity effect of metabolites on SW480 cells. Apoptosis and cell cycle arrests were measured by flowcytometry. Morphological changes were indicated by Propidium iodide staining andP53 gene expression was evaluated by real-time PCR. Streptomyces Levis ABRIINW111 inhibited cell growth, increased Caspases 3 and reduced Ki67 expression in a concentration/time-dependent manner in SW40 cells. Metabolites increased subG1 phase (apoptosis) and also cell cycle arrest in G1, G---2/M and S phase. P53 gene expression followed Sw480 cells treatment significantly. Streptomyces sp. metabolites have anti-carcinogenic effect on colon cancer cells. Streptomyces Levis ABRIINW111 metabolites are a candidate for Colon cancer treatment.
链霉菌属是放线菌中的优势属,是多种次级代谢产物的来源。微生物代谢产物可作为新型抗癌剂,副作用较少。本文阐述了从链霉菌中提取的醚溶性有机代谢产物对SW480结肠癌细胞系的抗癌作用。进行MTT试验以研究代谢产物对SW480细胞的细胞毒性作用。通过流式细胞术检测细胞凋亡和细胞周期阻滞情况。用碘化丙啶染色显示形态学变化,通过实时PCR评估P53基因表达。链霉菌Levis ABRIINW111在SW40细胞中以浓度/时间依赖性方式抑制细胞生长,增加半胱天冬酶3的表达并降低Ki67的表达。代谢产物增加了亚G1期(凋亡)以及G1、G2/M和S期的细胞周期阻滞。P53基因表达在SW480细胞处理后显著变化。链霉菌属的代谢产物对结肠癌细胞具有抗癌作用。链霉菌Levis ABRIINW111的代谢产物是结肠癌治疗的候选药物。