School of Food Science and Technology, National Engineering Research Center of Seafood , Dalian Polytechnic University , 1 Qinggongyuan , Dalian , Liaoning 116034 , People's Republic of China.
Division of Biotechnology, Dalian Institute of Chemical Physics , Chinese Academy of Sciences , 457 Zhongshan Road , Dalian , Liaoning 116023 , People's Republic of China.
J Agric Food Chem. 2019 Jan 30;67(4):1156-1164. doi: 10.1021/acs.jafc.8b04667. Epub 2019 Jan 14.
Nine transformants of Rhodosporidium toruloides with significant changes in the carotenoid profile were obtained by Agrobacterium tumefaciens-mediated transformation, including a white, three red, and four yellow mutants. A red mutant A1-15-BRQ that showed a high torularhodin production was selected for culture condition optimization. Results indicated that the torularhodin yield was boosted with glucose as the carbon source, at a carbon/nitrogen ratio of 22, a loading volume of 75 mL, and 28 °C. The torularhodin yield of 21.3 mg/L consisting of 94.4% total carotenoids was obtained by Box-Behnken design experiments. The torularhodin yield was 17.0-fold higher than that of the wild type, with time shortened from 9 to 3 days. This study reports an effective strategy for improving torularhodin production and provides a candidate R. toruloides strain for highly selective production of torularhodin.
通过农杆菌介导转化，获得了 9 株类球红酵母的转化子，这些转化子的类胡萝卜素图谱发生了显著变化，包括 1 株白色、3 株红色和 4 株黄色突变体。选择了一个红色突变体 A1-15-BRQ，其 torularhodin 产量较高，用于培养条件优化。结果表明，以葡萄糖为碳源、碳氮比为 22、装液量为 75 mL、温度为 28°C 时，torularhodin 的产量得到了提高。通过 Box-Behnken 设计实验，获得了 21.3 mg/L 的 torularhodin 产量，其中总类胡萝卜素含量为 94.4%。torularhodin 的产量比野生型提高了 17.0 倍，时间从 9 天缩短到 3 天。本研究报道了一种提高 torularhodin 产量的有效策略，并为高选择性生产 torularhodin 提供了一个候选的类球红酵母菌株。
