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Design, synthesis and expression of a human interleukin-2 gene incorporating the codon usage bias found in highly expressed Escherichia coli genes.

作者信息

Williams D P, Regier D, Akiyoshi D, Genbauffe F, Murphy J R

机构信息

Evans Department of Clinical Research, University Hospital, Boston, MA.

出版信息

Nucleic Acids Res. 1988 Nov 25;16(22):10453-67. doi: 10.1093/nar/16.22.10453.

DOI:10.1093/nar/16.22.10453
PMID:3060845
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC338917/
Abstract

A synthetic gene encoding human interleukin-2 (IL-2) was designed such that the codon usage bias resembled that found in highly expressed Escherichia coli genes. The percentage of preferred codons was increased from 43% in the native cDNA sequence to 85% in the synthetic sequence. The cDNA and synthetic IL-2 genes were placed under the control of the trc promoter and expressed in E. coli JM101. While Northern blot analysis of IL-2 mRNA from each genetic construct demonstrated equivalent message half-lives, immunoblot and bioactivity analyses showed the synthetic gene to direct the synthesis of up to 16 times more IL-2 than the native cDNA sequence.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5ed/338917/adf300cd0470/nar00164-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5ed/338917/3c428ae315f1/nar00164-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5ed/338917/ada57fd5b0d3/nar00164-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5ed/338917/adf300cd0470/nar00164-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5ed/338917/3c428ae315f1/nar00164-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5ed/338917/ada57fd5b0d3/nar00164-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5ed/338917/adf300cd0470/nar00164-0066-a.jpg

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1
Design, synthesis and expression of a human interleukin-2 gene incorporating the codon usage bias found in highly expressed Escherichia coli genes.
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2
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4
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Thermostable proteins bioprocesses: The activity of restriction endonuclease-methyltransferase from Thermus thermophilus (RM.TthHB27I) cloned in Escherichia coli is critically affected by the codon composition of the synthetic gene.耐热蛋白质生物加工：克隆在大肠杆菌中的耐热栖热菌（Thermus thermophilus）限制内切酶-甲基转移酶（RM.TthHB27I）的活性受到合成基因密码子组成的严重影响。
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[Regularities of the nucleotide sequence at the 5'-end of the codon in Escherichia coli genes].[大肠杆菌基因密码子5′端核苷酸序列的规律]
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Nucleic Acids Res. 1986 Oct 10;14(19):7737-49. doi: 10.1093/nar/14.19.7737.

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A new and updated resource for codon usage tables.

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T-cell growth factor.T细胞生长因子。
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Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes.原核基因中的密码子偏好使用：最佳密码子 - 反密码子相互作用能量与高效表达基因中的选择性密码子使用
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Structure and expression of a cloned cDNA for human interleukin-2.人白细胞介素-2克隆cDNA的结构与表达
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Gene optimization mechanisms: a multi-gene study reveals a high success rate of full-length human proteins expressed in Escherichia coli.基因优化机制：一项多基因研究表明，在大肠杆菌中表达全长人蛋白的成功率很高。
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Mycobacterial codon optimization enhances antigen expression and virus-specific immune responses in recombinant Mycobacterium bovis bacille Calmette-Guérin expressing human immunodeficiency virus type 1 Gag.分枝杆菌密码子优化增强了表达人免疫缺陷病毒1型Gag的重组卡介苗中抗原的表达及病毒特异性免疫反应。
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How optimized is the translational machinery in Escherichia coli, Salmonella typhimurium and Saccharomyces cerevisiae?大肠杆菌、鼠伤寒沙门氏菌和酿酒酵母中的翻译机制有多优化？
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Synonymous codon usage in Zea mays L. nuclear genes is varied by levels of C and G-ending codons.玉米（Zea mays L.）核基因中的同义密码子使用情况因以C和G结尾的密码子水平而异。
Nucleic Acids Res. 1993 Nov 25;21(23):5294-300. doi: 10.1093/nar/21.23.5294.
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The production of strains highly expressing human interleukin-2 cDNA.高表达人白细胞介素-2 cDNA 的菌株的产生。
J Tongji Med Univ. 1994;14(1):16-9, 41. doi: 10.1007/BF02888051.
9
Expression of tetanus toxin fragment C in E. coli: high level expression by removing rare codons.破伤风毒素片段C在大肠杆菌中的表达：通过去除稀有密码子实现高水平表达。
Nucleic Acids Res. 1989 Dec 25;17(24):10191-202. doi: 10.1093/nar/17.24.10191.
10
Overproduction and localization of components of the polyketide synthase of Streptomyces glaucescens involved in the production of the antibiotic tetracenomycin C.参与抗生素四环素霉素C产生的浅蓝链霉菌聚酮合酶组分的过量产生和定位。
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Biological activity of recombinant human interleukin-2 produced in Escherichia coli.在大肠杆菌中产生的重组人白细胞介素-2的生物活性。
Science. 1984 Mar 30;223(4643):1412-4. doi: 10.1126/science.6367046.
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Amino acid sequence and post-translational modification of human interleukin 2.人白细胞介素2的氨基酸序列与翻译后修饰
Proc Natl Acad Sci U S A. 1984 Oct;81(20):6486-90. doi: 10.1073/pnas.81.20.6486.
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Molecular cloning of human interleukin 2 cDNA and its expression in E. coli.人白细胞介素2 cDNA 的分子克隆及其在大肠杆菌中的表达。
Nucleic Acids Res. 1983 Jul 11;11(13):4307-23. doi: 10.1093/nar/11.13.4307.
7
Systematic alteration of the nucleotide sequence preceding the translation initiation codon and the effects on bacterial expression of the cloned SV40 small-t antigen gene.翻译起始密码子之前核苷酸序列的系统性改变及其对克隆的SV40小t抗原基因细菌表达的影响。
Gene. 1982 Jan;17(1):55-63. doi: 10.1016/0378-1119(82)90100-7.
8
Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts.大肠杆菌中mRNA的衰变：转录本特定片段命运的研究
Proc Natl Acad Sci U S A. 1983 Feb;80(3):653-7. doi: 10.1073/pnas.80.3.653.
9
Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: a proposal for a synonymous codon choice that is optimal for the E. coli translational system.大肠杆菌转移RNA丰度与其蛋白质基因中相应密码子出现情况之间的相关性：关于一种对大肠杆菌翻译系统而言最优的同义密码子选择的提议。
J Mol Biol. 1981 Sep 25;151(3):389-409. doi: 10.1016/0022-2836(81)90003-6.
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Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes.大肠杆菌转移RNA丰度与其蛋白质基因中相应密码子出现情况之间的相关性。
J Mol Biol. 1981 Feb 15;146(1):1-21. doi: 10.1016/0022-2836(81)90363-6.