University of Carthage, Biochemistry and Molecular Biology Lab of Faculty of Sciences, Risks Related to Environmental Stress, Struggle and Prevention (UR17ES20), Bizerte, Zarzouna, Tunisia.
Laboratory of Environmental Analytical Chemistry, University of Carthage, Faculty of Sciences of Bizerte, Zarzouna, Tunisia.
PLoS One. 2019 Jan 4;14(1):e0208431. doi: 10.1371/journal.pone.0208431. eCollection 2019.
One of the main challenges of bioremediation is to define efficient protocols with low environmental impact and high removal rates, such as static magnetic field (SMF). The aim of this study was to evaluate the effect of SMF exposure on the biodegradation rate of a mixture of pollutants using three bacterial strains which were isolated and identified from the Bizerte lagoon: Pseudomonas stutzeri LBR (KC157911), Cupriavidus metallidurans LBJ (KU659610) and Rhodococcus equi LBB (KU743870). To recognize the improvement role of SMF, the culture was submitted to a pre-treatment with SMF with an induction equal to 200 mT for 5 hours, after that the degradation experiment was followed with individual strains and with a consortium. Results showed an increase by 20% in the growth of the exposed bacterial population compared to controls, and 98% of biodegradation of DDT and 90% for BaP after 30 days of follow-up. This encouraging data opens new perspectives for a bioremediation bioprocess using SMF.
生物修复的主要挑战之一是定义具有低环境影响和高去除率的有效方案，例如静磁场（SMF）。本研究的目的是评估 SMF 暴露对从比塞大泻湖分离和鉴定的三种细菌菌株（施氏假单胞菌 LBR（KC157911）、金属铜绿假单胞菌 LBJ（KU659610）和马红球菌 LBB（KU743870））混合污染物生物降解率的影响。为了认识到 SMF 的改善作用，将培养物用等于 200 mT 的 SMF 进行预处理 5 小时，然后用单独的菌株和 consortium 进行降解实验。结果表明，与对照相比，暴露细菌种群的生长增加了 20%，并且在 30 天的跟踪后，DDT 的生物降解率达到了 98%，BaP 达到了 90%。这些令人鼓舞的数据为使用 SMF 的生物修复生物工艺开辟了新的前景。
