Aziz A, Agamuthu P, Alaribe F O, Fauziah S H
a Lasbela University of Agriculture, Water and Marine Sciences , Uthal , Pakistan.
b Institute of Biological Sciences, Faculty of Science , University of Malaya , Kuala Lumpur , Malaysia.
Environ Technol. 2018 Feb;39(4):527-535. doi: 10.1080/09593330.2017.1305455. Epub 2017 Mar 27.
Benzo[a]pyrene is a high-molecular-weight polycyclic aromatic hydrocarbon highly recalcitrant in nature and thus harms the ecosystem and/or human health. Therefore, its removal from the marine environment is crucial. This research focuses on benzo[a]pyrene degradation by using enriched bacterial isolates in consortium under saline conditions. Bacterial isolates capable of using benzo[a]pyrene as sole source of carbon and energy were isolated from enriched mangrove sediment. These isolates were identified as Ochrobactrum anthropi, Stenotrophomonas acidaminiphila, and Aeromonas salmonicida ss salmonicida. Isolated O. anthropi and S. acidaminiphila degraded 26% and 20%, respectively, of an initial benzo[a]pyrene concentration of 20 mg/L after 8 days of incubation in seawater (28 ppm of NaCl). Meanwhile, the bacterial consortium decomposed 41% of an initial 50 mg/L benzo[a]pyrene concentration after 8 days of incubation in seawater (28 ppm of NaCl). The degradation efficiency of benzo[a]pyrene increased to 54%, when phenanthrene was supplemented as a co-metabolic substrate. The order of biodegradation rate by temperature was 30°C > 25°C > 35°C. Our results suggest that co-metabolism by the consortium could be a promising biodegradation strategy for benzo[a]pyrene in seawater.
苯并[a]芘是一种高分子量的多环芳烃，在自然环境中具有高度抗性，因此会损害生态系统和/或人类健康。因此，从海洋环境中去除苯并[a]芘至关重要。本研究聚焦于在含盐条件下利用富集的混合细菌分离株降解苯并[a]芘。从富集的红树林沉积物中分离出能够将苯并[a]芘作为唯一碳源和能源的细菌分离株。这些分离株被鉴定为人苍白杆菌、嗜氨基寡养单胞菌和杀鲑气单胞菌杀鲑亚种。在海水（氯化钠含量为28 ppm）中培养8天后，分离出的人苍白杆菌和嗜氨基寡养单胞菌分别降解了初始浓度为20 mg/L的苯并[a]芘的26%和20%。同时，在海水（氯化钠含量为28 ppm）中培养8天后，混合细菌分解了初始浓度为50 mg/L的苯并[a]芘的41%。当添加菲作为共代谢底物时，苯并[a]芘的降解效率提高到了54%。按温度排序的生物降解速率为30°C＞25°C＞35°C。我们的结果表明，混合细菌的共代谢可能是海水中苯并[a]芘一种有前景的生物降解策略。
