Research Laboratory of Real Samples Analysis, Department of Analytical Chemistry, Faculty of Chemistry, Iran University of Science and Technology, P.O. Box 1684613114, Tehran, Iran.
Research Laboratory of Real Samples Analysis, Department of Analytical Chemistry, Faculty of Chemistry, Iran University of Science and Technology, P.O. Box 1684613114, Tehran, Iran.
Talanta. 2019 Mar 1;194:534-541. doi: 10.1016/j.talanta.2018.10.053. Epub 2018 Oct 24.
A new solid phase luminescence sensor (SPLS) based on molecularly imprinted polymer (MIP) as recognition element and semi-conductor CdSeS/ZnS alloyed quantum dots (QDs) as fluorophore has been synthesized and developed for the determination of sulfasalazine. The surface modified quantum dot was attached to the surface of the glass slide. MIP and Non-imprinted polymer (NIP) with the recognition site for Sulfasalazine (SSZ) were synthesized and attached to the immobilized QDs. A trace amount of sulfasalazine was determined using fluorescence spectroscopy method based on quenching effect of sulfasalazine on the QDs luminescence with a fluorescence resonance energy transfer (FRET) mechanism. The MIP-QDs based sensor showed high recognition capability of sulfasalazine in comparison with the NIP-QDs based sensor. Various experimental parameters affecting fluorescence intensity such as the reactions time and pH were investigated and optimized. Under optimal conditions, the MIP-QDs sensor showed good linearity for sulfasalazine in the range of 0.02-1.5 µmol L with a determination coefficient (R) of 0.9986. The limit of detection (S/N = 3) and limit of quantification (S/N = 10) were found to be 0.0071 and 0.024 µmol L, respectively. The intra-day and inter-day RSDs were 2.6-5.1% and 3.5-6.9%, respectively. The MIP-QDs based sensor was successfully used to the determination of sulfasalazine in human plasma and urine with the recoveries in the range of 90-107%, offering reliability and applicability of the sensor to the complex matrices.
一种基于分子印迹聚合物（MIP）作为识别元件和半导体 CdSeS/ZnS 合金量子点（QDs）作为荧光团的新型固相荧光传感器（SPLS）已被合成和开发，用于测定柳氮磺胺吡啶。经过表面修饰的量子点被附着在玻片表面。合成了具有磺胺嘧啶（SSZ）识别位点的印迹聚合物（MIP）和非印迹聚合物（NIP），并附着在固定化的 QDs 上。基于磺胺嘧啶对 QDs 荧光的猝灭效应，使用荧光光谱法测定痕量磺胺嘧啶，荧光共振能量转移（FRET）机制。与基于 NIP-QDs 的传感器相比，MIP-QDs 传感器对磺胺嘧啶具有更高的识别能力。研究并优化了影响荧光强度的各种实验参数，如反应时间和 pH 值。在最佳条件下，MIP-QDs 传感器对磺胺嘧啶在 0.02-1.5µmol•L 范围内表现出良好的线性关系，测定系数（R）为 0.9986。检测限（S/N=3）和定量限（S/N=10）分别为 0.0071 和 0.024µmol•L。日内和日间 RSD 分别为 2.6-5.1%和 3.5-6.9%。该 MIP-QDs 传感器成功用于人血浆和尿液中磺胺嘧啶的测定，回收率在 90-107%之间，证明了传感器对复杂基质的可靠性和适用性。
