Department of Plant Science and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
Plant Genomics and Breeding Institute, Seoul National University, Seoul 08826, Korea.
Int J Mol Sci. 2019 Jan 1;20(1):135. doi: 10.3390/ijms20010135.
Branch number is one of the main factors affecting the yield of soybean ( (L.)). In this study, we conducted a genome-wide association study combined with linkage analysis for the identification of a candidate gene controlling soybean branching. Five quantitative trait nucleotides (QTNs) were associated with branch numbers in a soybean core collection. Among these QTNs, a linkage disequilibrium (LD) block spanning 20 genes was found to overlap a previously identified major quantitative trait locus . To validate and narrow down , we developed a set of near-isogenic lines (NILs) harboring high-branching (HB) and low-branching (LB) alleles of , with 99.96% isogenicity and different branch numbers. A cluster of single nucleotide polymorphisms (SNPs) segregating between NIL-HB and NIL-LB was located within the LD block. Among the five genes showing differential expression between NIL-HB and NIL-LB, (; Glyma.06G210600) was down-regulated in the shoot apex of NIL-HB, and one missense mutation and two SNPs upstream of were associated with branch numbers in 59 additional soybean accessions. encodes TEOSINTE-BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTORS 1 and 2 transcription factor and functions as a regulatory repressor of branching. On the basis of these results, we propose as a candidate gene for branching in soybean.
分支数是影响大豆产量的主要因素之一。本研究通过全基因组关联分析结合连锁分析，鉴定出一个控制大豆分枝的候选基因。在大豆核心群体中发现了 5 个与分枝数相关的数量性状核苷酸 (QTN)。在这些 QTN 中，发现一个跨越 20 个基因的连锁不平衡 (LD) 块与先前鉴定的主要数量性状位点重叠。为了验证和缩小范围，我们开发了一组携带高分枝 (HB) 和低分枝 (LB) 等位基因的近等基因系 (NIL)，其同源性为 99.96%，分枝数不同。在 NIL-HB 和 NIL-LB 之间分离的一组单核苷酸多态性 (SNP) 位于 LD 块内。在 NIL-HB 和 NIL-LB 之间表达差异的五个基因中，(Glyma.06G210600)在 NIL-HB 的茎尖中下调，位于 上游的一个错义突变和两个 SNP 与 59 个额外大豆品系的分枝数相关。 编码 TEOSINTE-BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTORS 1 和 2 转录因子，作为分枝的调节抑制子。基于这些结果，我们提出 作为大豆分枝的候选基因。
