Campagne Sebastien, Krepl Miroslav, Sponer Jiri, Allain Frederic H-T
Department of Biology, ETH Zürich, Institute of Molecular Biology and Biophysics, Zürich, Switzerland.
Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic; Department of Physical Chemistry, Faculty of Science, Regional Centre of Advanced Technologies and Materials, Palacky University Olomouc, Olomouc, Czech Republic.
Methods Enzymol. 2019;614:393-422. doi: 10.1016/bs.mie.2018.09.002. Epub 2018 Dec 10.
Understanding the RNA binding specificity of protein is of primary interest to decipher their function in the cell. Here, we review the methodology used to solve the structures of protein-RNA complexes using solution-state NMR spectroscopy: from sample preparation to structure calculation procedures. We also describe how molecular dynamics simulations can help providing additional information on the role of key amino acid side chains and of water molecules in protein-RNA recognition.
了解蛋白质的RNA结合特异性对于阐明其在细胞中的功能至关重要。在此，我们综述了利用溶液核磁共振波谱法解析蛋白质-RNA复合物结构所使用的方法：从样品制备到结构计算程序。我们还描述了分子动力学模拟如何有助于提供关于关键氨基酸侧链和水分子在蛋白质-RNA识别中的作用的额外信息。
