1 Department of Food Safety and Nutrition, College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, China.
2 Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Microb Drug Resist. 2019 Jun;25(5):703-711. doi: 10.1089/mdr.2018.0175. Epub 2019 Jan 16.
Disinfectant resistance and biofilm formation capacity are two important characteristics that contribute to the persistence of microorganisms in food processing environments and contamination of food products. This study investigated the susceptibility of 510 isolates against 5 disinfectants and the prevalence of 10 disinfectant-resistant genes in these isolates. The biofilm formation capacity of 194 isolates was determined, and the correlation between disinfectant resistance and biofilm formation was analyzed. The minimal inhibitory concentrations (MICs) of cetyltrimethylammonium bromide (CTAB), benzalkonium chloride (BC), cetylpyridinium chloride, and chlorhexidine (CHX) against isolates were 32-512, 16-256, 32-256, and 2-32 mg/L, respectively. The MICs of triclosan against 88.43% of isolates were 8-1,024 mg/L, while the MICs for the rest of isolates exceed 2,048 mg/L. The presence of , , and genes was significantly correlated with the CHX resistance of isolates, while the presence of and genes was significantly correlated with CTAB and BC resistance, respectively. The biofilm formation capacity (adjusted optical density value) was positively correlated with BC resistance ( = 0.201, < 0.01) and showed no correlation with other disinfectants. The presence of was positively correlated with biofilm formation, while four genes were negatively correlated with biofilm formation. This study provides useful data on disinfectant resistance and biofilm formation capacity of contaminating poultry products, which could be helpful in guiding proper disinfectant usage and establishing effective biofilm eradication strategy in food industry.
消毒剂耐药性和生物膜形成能力是两个重要特征，它们有助于微生物在食品加工环境中的持久性和食品污染。本研究调查了 510 株分离株对 5 种消毒剂的敏感性以及这些分离株中 10 种消毒剂耐药基因的流行情况。测定了 194 株分离株的生物膜形成能力，并分析了消毒剂耐药性与生物膜形成之间的相关性。十六烷基三甲基溴化铵（CTAB）、苯扎氯铵（BC）、十六烷基吡啶氯盐和洗必泰（CHX）对分离株的最小抑菌浓度（MIC）分别为 32-512、16-256、32-256 和 2-32 mg/L。三氯生对 88.43%的分离株的 MIC 为 8-1024 mg/L，而其余分离株的 MIC 超过 2048 mg/L。 、 和 基因的存在与分离株对 CHX 的耐药性显著相关，而 和 基因的存在分别与 CTAB 和 BC 耐药性显著相关。生物膜形成能力（调整后的光密度值）与 BC 耐药性呈正相关（ = 0.201， < 0.01），与其他消毒剂无相关性。 基因的存在与生物膜形成呈正相关，而四个基因与生物膜形成呈负相关。本研究提供了有关污染家禽产品的消毒剂耐药性和生物膜形成能力的有用数据，这有助于指导在食品工业中正确使用消毒剂和建立有效的生物膜清除策略。
