Smirnova D N, Krupina K A, Bogacheva N V, Darmov I V
Klin Lab Diagn. 2017 Jan;62(1):30-4.
The article summarizes data of research studies concerning searching optimal conditions of development of highly specific and highly sensitive immune chromatography test-systems designed for detection of agents of infectious diseases. The analysis was implemented concerning choosing of optimal size of nano-particles of colloid gold and concentration of antibodies for production of conjugate. The alternatives are presented concerning the most frequently used combinations of buffer solutions applied for development and analysis of immune chromatography test-systems. The preferences are established in the field of characteristics of membranes used in composition of multi-membrane composite of immune chromatographic test-systems.
本文总结了有关研究的数据，这些研究旨在探寻用于检测传染病病原体的高特异性和高灵敏度免疫层析检测系统的最佳开发条件。针对用于制备缀合物的胶体金纳米颗粒的最佳尺寸和抗体浓度的选择进行了分析。给出了用于免疫层析检测系统开发和分析的最常用缓冲溶液组合的替代方案。在免疫层析检测系统多膜复合物组成中使用的膜的特性方面确定了偏好。
